CoraLite® Plus 647 Anti-Rat CD45RA (OX-33) Mouse IgG2a Recombinant Antibody

CD45RA Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

rat

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

OX-33

Cat no : CL647-65578

Print datasheet

Synonyms

Lca, Ptprc, CD45, RT7, OX-33



经过测试的应用

Positive FC detected inrat splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65578-1-MR

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-65578 targets CD45RA in FC applications and shows reactivity with rat samples.

Tested Applications FC Application Description
Tested Reactivity rat
Immunogen Leukocyte common antigen purified from rat splenocytes 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Full Name protein tyrosine phosphatase, receptor type, C
Synonyms Lca, Ptprc, CD45, RT7, OX-33
Calculated Molecular Weight143 kDa
GenBank Accession NumberXM_006249910
Gene Symbol CD45
Gene ID (NCBI) 24699
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signaling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 CD45RA antibody CL647-65578Download protocol
Standard Protocols
Click here to view our Standard Protocols