CoraLite® Plus 488 Anti-Human CD45RO (UCHL1)
CD45RO Monoclonal Antibody for IF/ICC, FC
Host / Isotype
Mouse / IgG2a, kappa
Reactivity
human
Applications
IF/ICC, FC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
UCHL1
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | human peripheral blood mononuclear cells |
| Positive FC detected in | human PBMCs |
Planning an IHC experiment? We recommend our IHCeasy CD45 Ready-To-Use IHC Kit. CD45 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65150-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:150-1:600 |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-65150 targets CD45RO in IF/ICC, FC applications and shows reactivity with human samples.
| Tested Applications | IF/ICC, FC Application Description |
| Tested Reactivity | human |
| Immunogen | IL-2 dependent T cell line, CA1 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | protein tyrosine phosphatase, receptor type, C |
| Synonyms | CD45, B220, L CA, GP180, EC:3.1.3.48 |
| GenBank Accession Number | BC014239 |
| Gene Symbol | CD45 |
| Gene ID (NCBI) | 5788 |
| ENSEMBL Gene ID | ENSG00000081237 |
| RRID | AB_2883207 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | P08575 |
| Storage Buffer | PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RO is the shortest CD45 isoform (lacking all three exons) and is expressed by activated and memory T cells, thymocytes, some B cells, and weakly on granulocytes and macrophage (PMID: 27100180; 12414720).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 CD45RO antibody CL488-65150 | Download protocol |
| FC protocol for CL Plus 488 CD45RO antibody CL488-65150 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
