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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 84599-5-RR (same clone as 84599-5-PBS)

    WB analysis using 84599-5-RR (same clone as 84599-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84599-5-RR (CD46 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84599-5-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 84599-5-RR (same clone as 84599-5-PBS)

    IHC staining of human colon cancer using 84599-5-RR (same clone as 84599-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84599-5-RR (CD46 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84599-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD46 antibody included
  • IF Staining of HeLa using 84599-5-RR (same clone as 84599-5-PBS)

    IF Staining of HeLa using 84599-5-RR (same clone as 84599-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CD46 antibody (84599-5-RR, Clone: 241764F8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84599-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84599-5-RR

    Affinity and Kinetic Characterization of 84599-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84599-5-RR against Human CD46 were performed. The affinity constant is below 1 pM.

CD46 Recombinant antibody, PBS Only

CD46 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 84599-5-PBS

产品说明书

CloneNo. 241764F8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

TRA2.10, TLX, MIC10, Membrane cofactor protein, MCP

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CD46 Ready-To-Use IHC Kit. CD46 primary antibody included.

产品信息

84599-5-PBS targets CD46 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD46 molecule, complement regulatory protein
别名 TRA2.10, TLX, MIC10, Membrane cofactor protein, MCP
计算分子量42kDa
观测分子量50-65 kDa
GenBank蛋白编号NM_172351.3
基因名称 CD46
Gene ID (NCBI) 4179
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP15529-11
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD46, also known as Membrane cofactor protein (MCP) or MIC10, is a ubiquitously expressed type type-I transmembrane glycoprotein that acts as a cofactor for complement factor I to mediate inactivation of C3b and C4b deposited on host cells. CD46 is a receptor for a large number of pathogens, including measles virus and adenovirus (PMID: 20941397). CD46 plays a role in linking innate and adaptive immune responses. It may be involved in the fusion of the spermatozoa with the oocyte during fertilization. Mutations of the CD46 gene have been associated with some diseases including hemolytic uremic syndrome (PMID: 26054645). CD46 is expressed on most cells as four isoforms that are derived via alternative splicing (PMID: 15324743).