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CD46 Recombinant antibody, PBS Only

CD46 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241764F8

Cat no : 84599-5-PBS

Print datasheet

Synonyms

TRA2.10, TLX, MIC10, Membrane cofactor protein, MCP

验证数据展示

  • WB analysis using 84599-5-RR (same clone as 84599-5-PBS)

    WB analysis using 84599-5-RR (same clone as 84599-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84599-5-RR (CD46 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84599-5-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84599-5-RR (same clone as 84599-5-PBS)

    IF Staining of HeLa using 84599-5-RR (same clone as 84599-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CD46 antibody (84599-5-RR, Clone: 241764F8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84599-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84599-5-RR

    Affinity and Kinetic Characterization of 84599-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84599-5-RR against Human CD46 were performed. The affinity constant is below 1 pM.

Planning an IHC experiment? We recommend our IHCeasy CD46 Ready-To-Use IHC Kit. CD46 primary antibody included.

产品信息

84599-5-PBS targets CD46 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD46 molecule, complement regulatory protein
Synonyms TRA2.10, TLX, MIC10, Membrane cofactor protein, MCP
Calculated Molecular Weight42kDa
Observed Molecular Weight50-65 kDa
GenBank Accession NumberNM_172351.3
Gene Symbol CD46
Gene ID (NCBI) 4179
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP15529-11
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD46, also known as Membrane cofactor protein (MCP) or MIC10, is a ubiquitously expressed type type-I transmembrane glycoprotein that acts as a cofactor for complement factor I to mediate inactivation of C3b and C4b deposited on host cells. CD46 is a receptor for a large number of pathogens, including measles virus and adenovirus (PMID: 20941397). CD46 plays a role in linking innate and adaptive immune responses. It may be involved in the fusion of the spermatozoa with the oocyte during fertilization. Mutations of the CD46 gene have been associated with some diseases including hemolytic uremic syndrome (PMID: 26054645). CD46 is expressed on most cells as four isoforms that are derived via alternative splicing (PMID: 15324743).