验证数据展示
经过测试的应用
| Positive IF/ICC detected in | A549 cells, Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL532-66304 targets CD47 in IF/ICC applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human |
| 免疫原 | CD47 fusion protein Ag14132 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | CD47 molecule |
| 别名 | Leukocyte surface antigen CD47, Integrin-associated protein, Integrin associated protein, IAP, CD47 molecule |
| 计算分子量 | 323 aa, 35 kDa |
| 观测分子量 | 50-55 kDa |
| GenBank蛋白编号 | BC010016 |
| 基因名称 | CD47 |
| Gene ID (NCBI) | 961 |
| 偶联类型 | CoraLite®532 Fluorescent Dye |
| 最大激发/发射波长 | 537 nm / 560 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q08722 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD47, also known as integrin-associated protein (IAP), is a member of the immunoglobulin superfamily containing a five-pass transmembrane attachment. CD47 is heavily glycosylated and widely expressed by hematopoietic and nonhematopoietic cells. CD47 interacts with several membrane integrins and also acts a receptor for thrombospondin (THBS1). It is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. CD47 also functions as a ligand for signal regulatory protein-α (SIRPα). Upon binding CD47, SIRPα initiates a signaling cascade that results in the inhibition of phagocytosis.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL532 CD47 antibody CL532-66304 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |