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验证数据展示

  • IF Staining of A549 using CL532-66304

    IF Staining of A549 using CL532-66304

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite®532 CD47 antibody (CL532-66304, Clone: 1E1D8 ) at dilution of 1:200.

  • IF Staining of Jurkat using CL532-66304

    IF Staining of Jurkat using CL532-66304

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CoraLite®532 CD47 antibody (CL532-66304, Clone: 1E1D8 ) at dilution of 1:200.

  • Featured Product
  • KD/KO Validated

CoraLite®532-conjugated CD47 Monoclonal antibody

CD47 Monoclonal Antibody for IF/ICC
Cat No. CL532-66304

Print datasheet

CloneNo. 1E1D8

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC

Synonyms

Leukocyte surface antigen CD47, Integrin-associated protein, Integrin associated protein, IAP, CD47 molecule

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®532
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA549 cells, Jurkat cells
For other applications, we recommend the unconjugated version of this antibody, 66304-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL532-66304 targets CD47 in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen CD47 fusion protein Ag14132 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name CD47 molecule
Synonyms Leukocyte surface antigen CD47, Integrin-associated protein, Integrin associated protein, IAP, CD47 molecule
Calculated Molecular Weight 323 aa, 35 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC010016
Gene Symbol CD47
Gene ID (NCBI) 961
Conjugate CoraLite®532 Fluorescent Dye
Excitation/Emission Maxima Wavelengths537 nm / 560 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ08722
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD47, also known as integrin-associated protein (IAP), is a member of the immunoglobulin superfamily containing a five-pass transmembrane attachment. CD47 is heavily glycosylated and widely expressed by hematopoietic and nonhematopoietic cells. CD47 interacts with several membrane integrins and also acts a receptor for thrombospondin (THBS1). It is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. CD47 also functions as a ligand for signal regulatory protein-α (SIRPα). Upon binding CD47, SIRPα initiates a signaling cascade that results in the inhibition of phagocytosis.

实验方案

Product Specific Protocols
IF protocol for CL532 CD47 antibody CL532-66304Download protocol
Standard Protocols
Click here to view our Standard Protocols