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Anti-Human CD49b (AK7) Mouse IgG2a Recombinant Antibody

CD49b Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

AK7

Cat no : 65583-1-MR

Print datasheet

Synonyms

ITGA2, Integrin alpha 2, GPIa, Collagen receptor, BR

验证数据展示

  • FC experiment of human peripheral blood platelets using 65583-1-MR

    FC experiment of human peripheral blood platelets using 65583-1-MR

    1x10^6 human peripheral blood platelets were surface stained with 0.25 ug Anti-Human CD49b (AK7) Mouse IgG2a Recombinant Antibody (65583-1-MR, Clone: AK7) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue), and PE-conjugated Goat Anti-Mouse IgG. Cells were not fixed.

经过测试的应用

Positive FC detected inhuman peripheral blood platelets

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65583-1-MR targets CD49b in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Human platelets 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Full Name integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor)
Synonyms ITGA2, Integrin alpha 2, GPIa, Collagen receptor, BR
Calculated Molecular Weight 126 kDa
GenBank Accession NumberNM_002203
Gene Symbol Integrin alpha 2
Gene ID (NCBI) 3673
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD49b, also known as alpha2 integrin, is a type I transmembrane glycoprotein which forms an adhesion molecule VLA-2 with beta1 integrin. CD49b is a receptor for many (extracellular) matrix and non-matrix molecules (PMID: 23847623). CD49b is expressed on numerous cell types including platelets, activated T cells, B cells, and monocytes. CD49b activation skews NK cell effector function to a phenotype that exhibits elevated IFN-γ production with a concomitant inhibition of motility and cytolytic function (PMID: 32971468, 32762681). The Anti-Human CD49b antibody, clone AK7, is derived from Human platelets.

实验方案

Product Specific Protocols
FC protocol for CD49b antibody 65583-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols