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Anti-Human CD5 (L17F12) Mouse IgG2a Recombinant Antibody

CD5 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

L17F12

Cat no : 65599-1-MR

Print datasheet

Synonyms

CD5, CD5 molecule, LEU1, Lymphocyte antigen T1/Leu 1, T1

验证数据展示

  • FC experiment of human PBMCs using 65599-1-MR

    FC experiment of human PBMCs using 65599-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD5 Mouse IgG2a Recombinant Antibody (65599-1-MR, Clone: L17F12) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using 65599-1-MR

    FC experiment of human PBMCs using 65599-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD5 Mouse IgG2a Recombinant Antibody (65599-1-MR, Clone:L17F12) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated Donkey Anti-Mouse IgG(H+L). Cells were not fixed. Lymphocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65599-1-MR targets CD5 in FC applications and shows reactivity with Human samples.

Tested Applications FC Application Description
Tested Reactivity Human
Immunogen Human T-cell acute lymphoblastic leukemia (T-ALL) cells 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Full Name CD5 molecule
Synonyms CD5, CD5 molecule, LEU1, Lymphocyte antigen T1/Leu 1, T1
Calculated Molecular Weight 495 aa, 55 kDa
GenBank Accession NumberBC027901
Gene Symbol CD5
Gene ID (NCBI) 921
ENSEMBL Gene IDENSG00000110448
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).

实验方案

Product Specific Protocols
FC protocol for CD5 antibody 65599-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols