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Mouse CD5 Recombinant antibody, PBS Only (Capture/Detector)

CD5 Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240244F10

Cat no : 83451-2-PBS

Print datasheet

Synonyms

Lyt 1, Ly A, Ly 12, Ly 1, CD5 antigen

验证数据展示

  • Sandwich ELISA standard curve of MP00448-2

    Sandwich ELISA standard curve of MP00448-2

    Sandwich ELISA standard curve of MP00448-2, Mouse CD5 Recombinant Matched Antibody Pair - PBS only. 83451-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0899. 83451-2-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

  • Sample test of MP00448-2

    Sample test of MP00448-2

    The mean CD5 concentration was determined to be 502.9 ng/mL in EL-4 cell extract based on a 5.2 mg/mL extract load, 111.7 ng/mL in mouse spleen cell extract based on a 4.8 mg/mL extract load and 314.2 ng/mL in mouse thymus cell extract based on a 6 mg/mL extract load.

  • Cytometric bead array standard curve of MP00448-2

    Cytometric bead array standard curve of MP00448-2

    Cytometric bead array standard curve of MP00448-2, MOUSE CD5 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83451-1-PBS. Detection antibody: 83451-2-PBS. Standard: Eg0899. Range: 0.156-20 ng/mL.

  • Cytometric bead array standard curve of MP00448-3

    Cytometric bead array standard curve of MP00448-3

    Cytometric bead array standard curve of MP00448-3, MOUSE CD5 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83451-2-PBS. Detection antibody: 83451-3-PBS. Standard: Eg0899. Range: 0.156-20 ng/mL.

产品信息

83451-2-PBS targets CD5 as part of a matched antibody pair:

MP00448-2: 83451-1-PBS capture and 83451-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP00448-3: 83451-2-PBS capture and 83451-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity mouse
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD5 antigen
Synonyms Lyt 1, Ly A, Ly 12, Ly 1, CD5 antigen
Calculated Molecular Weight54 kDa
GenBank Accession NumberNM_007650.3
Gene Symbol Cd5
Gene ID (NCBI) 12507
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP13379
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).