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验证数据展示

  • FC experiment of human PBMCs using Biotin-65609

    FC experiment of human PBMCs using Biotin-65609

    1x10^6 human PBMCs were surface stained with 5 ul Biotin Plus Anti-Human CD5 (UCHT2) Mouse IgG2a RecAb (Biotin-65609, Clone: UCHT2), or unstained cells. Cells were co-stained with CoraLite® Plus 647 Anti-Human CD19 (4G7) Mouse IgG2a Recombinant Antibody (CL647-65562, Clone: 4G7). Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using Biotin-65609

    FC experiment of human PBMCs using Biotin-65609

    1x10^6 human PBMCs were surface stained with 5 ul Biotin Plus Anti-Human CD5 (UCHT2) Mouse IgG2a RecAb (Biotin-65609, Clone: UCHT2) (red), or unstained cells (blue). Cells were not fixed. Lymphocytes were gated.

Biotin Anti-Human CD5 (UCHT2) Mouse IgG2a Recombinant Antibody

CD5 Uni-rAbTM Recombinant Antibody for FC
Cat No. Biotin-65609

产品说明书

CloneNo. UCHT2

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

FC

别名

T-cell surface glycoprotein CD5, T1, Lymphocyte antigen T1/Leu-1, Lymphocyte antigen T1/Leu 1, LEU1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Biotin
规格: 

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经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-65609 targets CD5 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 N/A 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Recombinant
产品类型 Antibody
全称 CD5 molecule
别名 T-cell surface glycoprotein CD5, T1, Lymphocyte antigen T1/Leu-1, Lymphocyte antigen T1/Leu 1, LEU1
计算分子量 495 aa, 55 kDa
GenBank蛋白编号BC027901
基因名称 CD5
Gene ID (NCBI) 921
ENSEMBL Gene IDENSG00000110448
偶联类型 Biotin
形式 Liquid
纯化方式Protein A purification
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).

实验方案

Product Specific Protocols
FC protocol for Biotin CD5 antibody Biotin-65609Download protocol
Standard Protocols
Click here to view our Standard Protocols