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CoraLite® Plus 405 Anti-Human CD5 (L17F12)

CD5 Monoclonal Antibody for FC

Host / Isotype

Mouse / Mouse IgG2a, kappa

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 405 Fluorescent Dye

CloneNo.

L17F12

Cat no : CL405-65200

Print datasheet

Synonyms

CD5, CD5 molecule, LEU1, Lymphocyte antigen T1/Leu 1, T1

验证数据展示

  • FC experiment of human PBMCs using CL405-65200

    FC experiment of human PBMCs using CL405-65200

    1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD5 (CL405-65200, Clone: L17F12) or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using CL405-65200

    FC experiment of human PBMCs using CL405-65200

    1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD5 (CL405-65200, Clone: L17F12) or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs
For other applications, we recommend the unconjugated version of this antibody, 65200-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL405-65200 targets CD5 in FC applications and shows reactivity with Human samples.

Tested Applications FC Application Description
Tested Reactivity Human
Immunogen Human T-cell acute lymphoblastic leukemia (T-ALL) cells 种属同源性预测
Host / Isotype Mouse / Mouse IgG2a, kappa
Class Monoclonal
Type Antibody
Full Name CD5 molecule
Synonyms CD5, CD5 molecule, LEU1, Lymphocyte antigen T1/Leu 1, T1
Calculated Molecular Weight 495 aa, 55 kDa
GenBank Accession NumberBC027901
Gene Symbol CD5
Gene ID (NCBI) 921
ENSEMBL Gene IDENSG00000110448
Conjugate CoraLite® Plus 405 Fluorescent Dye
Excitation/Emission Maxima Wavelengths399 nm / 422 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP06127
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).

实验方案

Product Specific Protocols
FC protocol for CL Plus 405 CD5 antibody CL405-65200Download protocol
Standard Protocols
Click here to view our Standard Protocols