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CoraLite® Plus 488-conjugated CD5 Recombinant antibody

CD5 Uni-rAbTM Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

240428A12

Cat no : CL488-83543-6

Print datasheet

Synonyms

T1, Lymphocyte antigen T1/Leu-1, Lymphocyte antigen T1/Leu 1, LEU1, CD5 molecule

验证数据展示

  • IF Staining of Jurkat using CL488-83543-6

    IF Staining of Jurkat using CL488-83543-6

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CoraLite® Plus 488 CD5 antibody (CL488-83543-6, Clone: 240428A12 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 83543-6-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-83543-6 targets CD5 in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD5 molecule
Synonyms T1, Lymphocyte antigen T1/Leu-1, Lymphocyte antigen T1/Leu 1, LEU1, CD5 molecule
Calculated Molecular Weight55 kDa
GenBank Accession NumberNM_014207.4
Gene Symbol CD5
Gene ID (NCBI) 921
ENSEMBL Gene IDENSG00000110448
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP06127
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CD5 antibody CL488-83543-6Download protocol
Standard Protocols
Click here to view our Standard Protocols