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CoraLite®594-conjugated CD5 Monoclonal antibody

CD5 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

4D12C10

Cat no : CL594-67278

Print datasheet

Synonyms

CD5, CD5 molecule, LEU1, Lymphocyte antigen T1/Leu 1, T1

验证数据展示

  • IF Staining of human tonsillitis using CL594-67278

    IF Staining of human tonsillitis using CL594-67278

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD5 antibody (CL594-67278, Clone: 4D12C10 ) at dilution of 1:200.

  • IF Staining of human tonsillitis using CL594-67278

    IF Staining of human tonsillitis using CL594-67278

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 CD5 antibody (CL594-67278, Clone: 4D12C10 ) at dilution of 1:200.

经过测试的应用

Positive IF-P detected inhuman tonsillitis tissue
For other applications, we recommend the unconjugated version of this antibody, 67278-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67278 targets CD5 in IF-P applications and shows reactivity with Human samples.

Tested Applications IF-P Application Description
Tested Reactivity Human
Immunogen CD5 fusion protein Ag11065 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name CD5 molecule
Synonyms CD5, CD5 molecule, LEU1, Lymphocyte antigen T1/Leu 1, T1
Calculated Molecular Weight 495 aa, 55 kDa
GenBank Accession NumberBC027901
Gene Symbol CD5
Gene ID (NCBI) 921
ENSEMBL Gene IDENSG00000110448
RRIDAB_2920105
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP06127
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).

实验方案

Product Specific Protocols
IF protocol for CL594 CD5 antibody CL594-67278Download protocol
Standard Protocols
Click here to view our Standard Protocols