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CoraLite® Plus 647 Anti-Mouse CD5 Rabbit Recombinant Antibody

CD5 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

240244A7

Cat no : CL647-98038-2

Print datasheet

Synonyms

Lyt 1, Ly A, Ly 12, Ly 1, CD5 antigen

验证数据展示

  • FC experiment of mouse splenocytes using CL647-98038-2

    FC experiment of mouse splenocytes using CL647-98038-2

    1x10^6 mouse splenocytes were surface stained with CoraLite® Plus 488 Anti-Mouse CD3ε and 0.25 ug CoraLite® Plus 647 Anti-Mouse CD5 Rabbit Recombinant Antibody (CL647-98038-2, Clone:240244A7), or Isotype Control. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of mouse splenocytes using CL647-98038-2

    FC experiment of mouse splenocytes using CL647-98038-2

    1x10^6 mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse CD5 Rabbit Recombinant Antibody (CL647-98038-2, Clone:240244A7) (red), or Isotype Control (blue). Cells were not fixed.

经过测试的应用

Positive FC detected inmouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 98038-2-PBS

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-98038-2 targets CD5 in FC applications and shows reactivity with mouse samples.

Tested Applications FC Application Description
Tested Reactivity mouse
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD5 antigen
Synonyms Lyt 1, Ly A, Ly 12, Ly 1, CD5 antigen
Calculated Molecular Weight54 kDa
GenBank Accession NumberNM_007650.3
Gene Symbol Cd5
Gene ID (NCBI) 12507
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP13379
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 CD5 antibody CL647-98038-2Download protocol
Standard Protocols
Click here to view our Standard Protocols