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CoraLite® Plus 647 Anti-Mouse CD5 Rabbit Recombinant Antibody

CD5 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

240244G7

Cat no : CL647-98038

Print datasheet

Synonyms

验证数据展示

  • FC experiment of mouse splenocytes using CL647-98038

    FC experiment of mouse splenocytes using CL647-98038

    1x10^6 mouse splenocytes were surface stained with CoraLite® Plus 488 Anti-Mouse CD3ε and 0.25 ug CoraLite® Plus 647 Anti-Mouse CD5 Rabbit Recombinant Antibody (CL647-98038, Clone:240244G7), or Isotype Control. Cells were not fixed.

  • FC experiment of mouse splenocytes using CL647-98038

    FC experiment of mouse splenocytes using CL647-98038

    1x10^6 mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse CD5 Rabbit Recombinant Antibody (CL647-98038, Clone:240244G7)(red), or Isotype Control(blue). Cells were not fixed.

经过测试的应用

Positive FC detected inmouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 98038-1-PBS

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL647-98038 is CD5 Fusion Protein expressed in E. coli.

Tested Applications FC Application Description
Tested Reactivity mouse
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD5 antigen
Synonyms
Calculated Molecular Weight54 kDa
GenBank Accession NumberNM_007650.3
Gene Symbol Cd5
Gene ID (NCBI) 12507
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP13379
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD5 is a type I transmembrane glycoprotein of the scavenger receptor cysteine-rich family (PMID: 12403363). CD5 is expressed on a majority of thymocytes, mature T cells, B cell subsets, and peripheral blood dendritic cells (PMID: 9858516; 6156984; 10379049; 1384337). CD5 may act as a receptor in regulating T-cell proliferation. It functions as a negative regulator of TCR signaling during thymocyte development (PMID: 7542801).

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 CD5 antibody CL647-98038Download protocol
Standard Protocols
Click here to view our Standard Protocols