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验证数据展示

  • FC experiment of human PBMCs using APC-65075

    FC experiment of human PBMCs using APC-65075

    1x10^6 human PBMCs were surface stained with 5 ul APC Anti-Human CD54 (ICAM-1) (APC-65075, Clone:15.2) (red) or APC Mouse IgG1 Isotype Control (APC-65124, Clone: MOPC-21) (blue). Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes and monocytes were gated.

  • FC experiment of human PBMCs using APC-65075

    FC experiment of human PBMCs using APC-65075

    1x10^6 human PBMCs were surface stained with 5 ul APC Anti-Human CD54 (ICAM-1) (APC-65075, Clone:15.2) or APC Mouse IgG1 Isotype Control (APC-65124, Clone: MOPC-21). Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes and monocytes were gated.

APC Anti-Human ICAM-1/CD54 (15.2)

ICAM-1/CD54 Monoclonal Antibody for FC
Cat No. APC-65075

产品说明书

CloneNo. 15.2
引用文献(2)

宿主/亚型

Mouse / IgG1, kappa

种属反应性

human

应用

FC

别名

CD54 (ICAM-1), CD54, ICAM-1, ICAM1, ICAM 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  APC
规格: 

-/ -

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经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy ICAM-1 Ready-To-Use IHC Kit. ICAM-1 primary antibody included.

推荐稀释比

应用推荐稀释比
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

FCSee 1 publications below

产品信息

APC-65075 targets ICAM-1/CD54 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
文献引用应用FC
经测试反应性 human
免疫原 Rheumatoid synovial cells and human monocytes 种属同源性预测
宿主/亚型 Mouse / IgG1, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 intercellular adhesion molecule 1
别名 CD54 (ICAM-1), CD54, ICAM-1, ICAM1, ICAM 1
计算分子量 90 kDa
GenBank蛋白编号BC015969
基因名称 ICAM-1
Gene ID (NCBI) 3383
ENSEMBL Gene IDENSG00000090339
RRIDAB_2882974
偶联类型 APC Fluorescent Dye
最大激发/发射波长650 nm / 660 nm
形式 Liquid
纯化方式Affinity purification
UNIPROT IDP05362
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).

实验方案

Product Specific Protocols
FC protocol for APC ICAM-1/CD54 antibody APC-65075Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Adv Healthc Mater

An Erythrocyte Membrane-Derived Nanosystem for Efficient Reversal of Endothelial Injury in Sepsis

Authors - Yao Zhang
View Article
FC

Cell Signal

RelB promotes the migration and invasion of prostate cancer DU145 cells via exosomal ICAM1 in vitro.

Authors - Wenjing Li
View Article