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APC Anti-Human ICAM-1/CD54 (15.2)

ICAM-1/CD54 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC

Conjugate

APC Fluorescent Dye

CloneNo.

15.2

Cat no : APC-65075

Print datasheet

Synonyms

CD54 (ICAM-1), CD54, ICAM-1, ICAM1, ICAM 1

验证数据展示

  • FC experiment of human PBMCs using APC-65075

    FC experiment of human PBMCs using APC-65075

    1x10^6 human PBMCs were surface stained with 5 ul APC Anti-Human CD54 (ICAM-1) (APC-65075, Clone:15.2) (red) or APC Mouse IgG1 Isotype Control (APC-65124, Clone: MOPC-21) (blue). Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes and monocytes were gated.

  • FC experiment of human PBMCs using APC-65075

    FC experiment of human PBMCs using APC-65075

    1x10^6 human PBMCs were surface stained with 5 ul APC Anti-Human CD54 (ICAM-1) (APC-65075, Clone:15.2) or APC Mouse IgG1 Isotype Control (APC-65124, Clone: MOPC-21). Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes and monocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy ICAM-1 Ready-To-Use IHC Kit. ICAM-1 primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

FCSee 1 publications below

产品信息

APC-65075 targets ICAM-1/CD54 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Cited ApplicationsFC
Tested Reactivity human
Immunogen Rheumatoid synovial cells and human monocytes 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name intercellular adhesion molecule 1
Synonyms CD54 (ICAM-1), CD54, ICAM-1, ICAM1, ICAM 1
Calculated Molecular Weight 90 kDa
GenBank Accession NumberBC015969
Gene Symbol ICAM-1
Gene ID (NCBI) 3383
ENSEMBL Gene IDENSG00000090339
RRIDAB_2882974
Conjugate APC Fluorescent Dye
Excitation/Emission Maxima Wavelengths650 nm / 660 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP05362
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).

实验方案

Product Specific Protocols
FC protocol for APC ICAM-1/CD54 antibody APC-65075Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Adv Healthc Mater

An Erythrocyte Membrane-Derived Nanosystem for Efficient Reversal of Endothelial Injury in Sepsis

Authors - Yao Zhang
View Article
FC

Cell Signal

RelB promotes the migration and invasion of prostate cancer DU145 cells via exosomal ICAM1 in vitro.

Authors - Wenjing Li
View Article