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CoraLite® Plus 488 Anti-Human ICAM-1/CD54 (15.2)

ICAM-1/CD54 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

15.2

Cat no : CL488-65075

Print datasheet

Synonyms

CD54 (ICAM-1), CD54, ICAM-1, ICAM1, ICAM 1

验证数据展示

  • FC experiment of human PBMCs using CL488-65075

    FC experiment of human PBMCs using CL488-65075

    1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD54 (ICAM-1) (CL488-65075, Clone:15.2) or unstained. Cells were not fixed. Monocytes were gated.

  • FC experiment of human PBMCs using CL488-65075

    FC experiment of human PBMCs using CL488-65075

    1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD54 (ICAM-1) (CL488-65075, Clone:15.2) or unstained. Cells were not fixed. Monocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy ICAM-1 Ready-To-Use IHC Kit. ICAM-1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 65075-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-65075 targets ICAM-1/CD54 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Rheumatoid synovial cells and human monocytes 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name intercellular adhesion molecule 1
Synonyms CD54 (ICAM-1), CD54, ICAM-1, ICAM1, ICAM 1
Calculated Molecular Weight 90 kDa
GenBank Accession NumberBC015969
Gene Symbol ICAM-1
Gene ID (NCBI) 3383
ENSEMBL Gene IDENSG00000090339
RRIDAB_2919254
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP05362
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 ICAM-1/CD54 antibody CL488-65075Download protocol
Standard Protocols
Click here to view our Standard Protocols