CoraLite® Plus 488 Anti-Mouse ICAM-1/CD54 (YN1/1.7.4)
ICAM-1/CD54 Monoclonal Antibody for FC
Host / Isotype
Rat / IgG2b, kappa
Reactivity
mouse
Applications
FC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
YN1/1.7.4
验证数据展示
经过测试的应用
| Positive FC detected in | mouse splenocytes |
For other applications, we recommend the unconjugated version of this antibody, 65066-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-65066 targets ICAM-1/CD54 in FC applications and shows reactivity with mouse samples.
| Tested Applications | FC Application Description |
| Tested Reactivity | mouse |
| Immunogen | Mouse NS-1 cells 种属同源性预测 |
| Host / Isotype | Rat / IgG2b, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | intercellular adhesion molecule 1 |
| Synonyms | CD54, Icam-1, YN1/1.7.4, MALA-2, Icam1 |
| GenBank Accession Number | BC008626 |
| Gene Symbol | ICAM-1 |
| Gene ID (NCBI) | 15894 |
| RRID | AB_2883159 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | P13597 |
| Storage Buffer | PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 ICAM-1/CD54 antibody CL488-65066 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
