CoraLite® Plus 488 Anti-Mouse CD54 (YN1/1.7.4)
CD54 Monoclonal Antibody for FC
Host / Isotype
Rat / IgG2b, kappa
Reactivity
mouse
Applications
FC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
YN1/1.7.4
验证数据展示
经过测试的应用
Positive FC detected in | mouse splenocytes |
For other applications, we recommend the unconjugated version of this antibody, 65066-1-Ig
推荐稀释比
Application | Dilution |
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This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-65066 targets CD54 in FC applications and shows reactivity with mouse samples.
Tested Applications | FC Application Description |
Tested Reactivity | mouse |
Immunogen | Mouse NS-1 cells 种属同源性预测 |
Host / Isotype | Rat / IgG2b, kappa |
Class | Monoclonal |
Type | Antibody |
Full Name | intercellular adhesion molecule 1 |
Synonyms | Icam-1, YN1/1.7.4, MALA-2, Icam1, ICAM 1 |
GenBank Accession Number | BC008626 |
Gene Symbol | ICAM-1 |
Gene ID (NCBI) | 15894 |
RRID | AB_2883159 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Affinity purification |
UNIPROT ID | P13597 |
Storage Buffer | PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3. |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
ICAM-1 (CD54) is a 90-kDa transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).
实验方案
Product Specific Protocols | |
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FC protocol for CL Plus 488 CD54 antibody CL488-65066 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |