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CD55 Recombinant antibody

CD55 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2D2

Cat no : 82781-6-RR

Print datasheet

Synonyms

TC, DAF, CROM, CR, Complement decay-accelerating factor

验证数据展示

  • WB analysis using 82781-6-RR

    WB analysis using 82781-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82781-6-RR (CD55 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 82781-6-RR

    IHC staining of human lung cancer using 82781-6-RR

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82781-6-RR (CD55 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 82781-6-RR

    IHC staining of human lung cancer using 82781-6-RR

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 82781-6-RR (CD55 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A549 using 82781-6-RR

    IF Staining of A549 using 82781-6-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CD55 antibody (82781-6-RR, Clone: 2D2 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Affinity and Kinetic Characterization of 82781-6-RR

    Affinity and Kinetic Characterization of 82781-6-RR

    Biolayer interferometry (BLl) kinetic assays of 82781-6-RR against Human CD55 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHeLa cells, A549 cells, MKN-45 cells, human placenta tissue, K-562 cells
Positive IHC detected inhuman lung cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82781-6-RR targets CD55 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen CD55 fusion protein Ag25026 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD55 molecule, decay accelerating factor for complement (Cromer blood group)
Synonyms TC, DAF, CROM, CR, Complement decay-accelerating factor
Calculated Molecular Weight 41 kDa
Observed Molecular Weight75 kDa
GenBank Accession NumberBC001288
Gene Symbol CD55
Gene ID (NCBI) 1604
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08174
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD55, also known as DAF, is a glycosylphosphatidylinositol (GPI)-anchored surface glycoprotein that is widely distributed on blood, stroma, epithelial, and endothelial cells (PMID: 7517044; 29503741). It can also exist as a soluble form in plasma, urine, saliva, tears, and synovial fluids (PMID: 29503741). CD55 is a complement regulatory protein (PMID: 2469439; 7517044). It inhibits formation of the C3 convertases through binding to C3b and C4b. It also binds the alternate pathway convertase C3bBb, the classical pathway convertase and C4b2a to accelerate their decay (PMID: 17289551). CD55 also serves as a receptor for coxsackieviruses B1, B3, and B5 and several enteroviruses (PMID: 7538177; 7517044). The observed molecular weight of mature CD55 varies between 50 to 100 kDa depending on the cell type. Different sizes of CD55 might be caused by alternative splicing or different glycosylation patterns (PMID: 29503741).

实验方案

Product Specific Protocols
WB protocol for CD55 antibody 82781-6-RRDownload protocol
IHC protocol for CD55 antibody 82781-6-RRDownload protocol
IF protocol for CD55 antibody 82781-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols