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Species Reactivity
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验证数据展示

  • WB analysis using 84519-4-RR

    WB analysis using 84519-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84519-4-RR (CD55 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

CD55 Recombinant antibody

CD55 Recombinant Antibody for WB, ELISA
Cat No. 84519-4-RR
CloneNo. 241808C10

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Synonyms

DAF-GPI, Daf1, Daf, Complement decay-accelerating factor, GPI-anchored, Cd55a

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue, mouse spleen tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84519-4-RR targets CD55 in WB, ELISA applications and shows reactivity with mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity mouse
Immunogen FusionProtein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD55 antigen
Synonyms DAF-GPI, Daf1, Daf, Complement decay-accelerating factor, GPI-anchored, Cd55a
Calculated Molecular Weight43kDa
Observed Molecular Weight58-70 kDa
GenBank Accession NumberNM_010016.3
Gene Symbol CD55
Gene ID (NCBI) 13136
RRIDAB_3672026
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ61475
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD55, also known as DAF, is a glycosylphosphatidylinositol (GPI)-anchored surface glycoprotein that is widely distributed on blood, stroma, epithelial, and endothelial cells (PMID: 7517044; 29503741). It can also exist as a soluble form in plasma, urine, saliva, tears, and synovial fluids (PMID: 29503741). CD55 is a complement regulatory protein (PMID: 2469439; 7517044). It inhibits formation of the C3 convertases through binding to C3b and C4b. It also binds the alternate pathway convertase C3bBb, the classical pathway convertase and C4b2a to accelerate their decay (PMID: 17289551). CD55 also serves as a receptor for coxsackieviruses B1, B3, and B5 and several enteroviruses (PMID: 7538177; 7517044). The observed molecular weight of mature CD55 varies between 50 to 100 kDa depending on the cell type. Different sizes of CD55 might be caused by alternative splicing or different glycosylation patterns (PMID: 29503741).

实验方案

Product Specific Protocols
WB protocol for CD55 antibody 84519-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols