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CoraLite® Plus 647 Anti-Human CD55 (JS11)

CD55 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

JS11

Cat no : CL647-65271

Print datasheet

Synonyms

CD55, CR, CROM, DAF, TC

验证数据展示

  • FC experiment of human PBMCs using CL647-65271

    FC experiment of human PBMCs using CL647-65271

    1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD55 (CL647-65271, Clone: JS11) (red) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using CL647-65271

    FC experiment of human PBMCs using CL647-65271

    1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD55 (CL647-65271, Clone: JS11) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs
For other applications, we recommend the unconjugated version of this antibody, 65271-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-65271 targets CD55 in FC applications and shows reactivity with Human samples.

Tested Applications FC Application Description
Tested Reactivity Human
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name CD55 molecule, decay accelerating factor for complement (Cromer blood group)
Synonyms CD55, CR, CROM, DAF, TC
Calculated Molecular Weight 41 kDa
GenBank Accession NumberBC001288
Gene Symbol CD55
Gene ID (NCBI) 1604
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD55, also known as DAF, is a glycosylphosphatidylinositol (GPI)-anchored surface glycoprotein that is widely distributed on blood, stroma, epithelial, and endothelial cells (PMID: 7517044; 29503741). It can also exist as a soluble form in plasma, urine, saliva, tears, and synovial fluids (PMID: 29503741). CD55 is a complement regulatory protein (PMID: 2469439; 7517044). It inhibits formation of the C3 convertases through binding to C3b and C4b. It also binds the alternate pathway convertase C3bBb, the classical pathway convertase and C4b2a to accelerate their decay (PMID: 17289551). CD55 also serves as a receptor for coxsackieviruses B1, B3, and B5 and several enteroviruses (PMID: 7538177; 7517044).

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 CD55 antibody CL647-65271Download protocol
Standard Protocols
Click here to view our Standard Protocols