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CoraLite® Plus 488-conjugated B3GAT1 Monoclonal antibody

B3GAT1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F4A11

Cat No : CL488-66711

Print datasheet

Synonyms

GlcAT-P, GLCATP, Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1, EC:2.4.1.135, Beta-1,3-glucuronyltransferase 1

验证数据展示

  • FC experiment of K-562 using CL488-66711

    FC experiment of K-562 using CL488-66711

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CD57 (CL488-66711, Clone:1F4A11) (red), or 0.4 ug CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inK-562 cells
For other applications, we recommend the unconjugated version of this antibody, 66711-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66711 targets B3GAT1 in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen B3GAT1 fusion protein Ag13593 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P)
Synonyms GlcAT-P, GLCATP, Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1, EC:2.4.1.135, Beta-1,3-glucuronyltransferase 1
Calculated Molecular Weight 38 kDa
Observed Molecular Weight 38 kDa, 100 kDa
GenBank Accession NumberBC010466
Gene Symbol B3GAT1
Gene ID (NCBI) 27087
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9P2W7
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

B3GAT1 is an enzyme encoded by the B3GAT1 gene, whose enzymatic activity generates the CD57 epitope on other cell surface proteins. In immunology, the CD57 antigen (CD stands for cluster of differentiation) is also known as HNK1 (human natural killer-1) or LEU7. B3GAT1 is associated with glycosaminoglycan biosynthesis. It plays a key role in neurodevelopment.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 B3GAT1 antibody CL488-66711Download protocol
Standard Protocols
Click here to view our Standard Protocols