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Anti-Human CD58 (TS2/9) Mouse IgG1 Recombinant Antibody

CD58 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

TS2/9

Cat no : 65517-1-MR

Print datasheet

Synonyms

Ag3, CD58, CD58 molecule, FLJ23181, FLJ43722, LFA 3, LFA3, Surface glycoprotein LFA 3

验证数据展示

  • FC experiment of human PBMCs using 65517-1-MR

    FC experiment of human PBMCs using 65517-1-MR

    1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD58 Mouse Recombinant Antibody (65517-1-MR, Clone:TS2/9) or Mouse IgG1 Isotype Control, and CoraLite®647-Conjugated Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with FITC-conjugated Anti-Human CD14. Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated.

  • FC experiment of human PBMCs using 65517-1-MR

    FC experiment of human PBMCs using 65517-1-MR

    1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD58 Mouse Recombinant Antibody (65517-1-MR, Clone:TS2/9) or Mouse IgG1 Isotype Control, and CoraLite®647-Conjugated Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65517-1-MR targets CD58 in FC applications and shows reactivity with Human samples.

Tested Applications FC Application Description
Tested Reactivity Human
Immunogen Human cytolytic T cells 种属同源性预测
Host / Isotype Mouse / IgG1
Class Recombinant
Type Antibody
Full Name CD58 molecule
Synonyms Ag3, CD58, CD58 molecule, FLJ23181, FLJ43722, LFA 3, LFA3, Surface glycoprotein LFA 3
Calculated Molecular Weight 28 kDa
GenBank Accession NumberBC005930
Gene Symbol CD58
Gene ID (NCBI) 965
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD58, also known as lymphocyte function-associated antigen 3 (LFA-3), is a heavily glycosylated protein of 55-70 kDa that is expressed on a broad range of hematopoietic and non-hematopoietic cells. CD58 is involved in immune recognition of tumor cells via binding of the CD2 receptor expressed on cytotoxic T cells.

实验方案

Product Specific Protocols
FC protocol for CD58 antibody 65517-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols