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验证数据展示

  • FC experiment of human PBMCs using 65517-1-PBS

    FC experiment of human PBMCs using 65517-1-PBS

    1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD58 Mouse Recombinant Antibody (65517-1-PBS, Clone:TS2/9) or Mouse IgG1 Isotype Control, and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with FITC-conjugated Anti-Human CD14. Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated.

  • FC experiment of human PBMCs using 65517-1-MR (same clone as 65517-1-PBS)

    FC experiment of human PBMCs using 65517-1-MR (same clone as 65517-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD58 Mouse Recombinant Antibody (65517-1-MR, Clone:TS2/9) or Mouse IgG1 Isotype Control, and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with FITC-conjugated Anti-Human CD14. Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated. This data was developed using the same antibody clone with 65517-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 65517-1-MR (same clone as 65517-1-PBS)

    FC experiment of human PBMCs using 65517-1-MR (same clone as 65517-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD58 Mouse Recombinant Antibody (65517-1-MR, Clone:TS2/9) or Mouse IgG1 Isotype Control, and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated. This data was developed using the same antibody clone with 65517-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 65517-1-PBS

    FC experiment of human PBMCs using 65517-1-PBS

    1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human CD58 Mouse Recombinant Antibody (65517-1-PBS, Clone:TS2/9) or Mouse IgG1 Isotype Control, and CoraLite®647-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Monocytes were gated.

Anti-Human CD58 (TS2/9) Mouse IgG1 Recombinant Antibody, PBS Only

CD58 Recombinant Antibody for FC
Cat No. 65517-1-PBS

产品说明书

CloneNo. TS2/9

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

FC

别名

Surface glycoprotein LFA 3, Lymphocyte function-associated antigen 3, LFA3, LFA 3, CD58 molecule

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65517-1-PBS targets CD58 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 Human cytolytic T cells 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Recombinant
产品类型 Antibody
全称 CD58 molecule
别名 Surface glycoprotein LFA 3, Lymphocyte function-associated antigen 3, LFA3, LFA 3, CD58 molecule
计算分子量 28 kDa
GenBank蛋白编号BC005930
基因名称 CD58
Gene ID (NCBI) 965
偶联类型 Unconjugated
形式 Liquid
纯化方式Affinity purification
储存缓冲液 PBS Only
储存条件Store at -80°C. Stable for one year after shipment.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD58, also known as lymphocyte function-associated antigen 3 (LFA-3), is a heavily glycosylated protein of 55-70 kDa that is expressed on a broad range of hematopoietic and non-hematopoietic cells. CD58 is involved in immune recognition of tumor cells via binding of the CD2 receptor expressed on cytotoxic T cells.

实验方案

Product Specific Protocols
FC protocol for CD58 antibody 65517-1-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols