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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
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Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • FC experiment of human peripheral blood leukocytes using 65597-1-MR

    FC experiment of human peripheral blood leukocytes using 65597-1-MR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD58 (TS2/9) Mouse IgG2a Recombinant Antibody (65597-1-MR, Clone: TS2/9) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) and PE-conjugated Goat Anti-Mouse IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human peripheral blood leukocytes using 65597-1-MR

    FC experiment of human peripheral blood leukocytes using 65597-1-MR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CD58 (TS2/9) Mouse IgG2a Recombinant Antibody (65597-1-MR, Clone: TS2/9) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue) and PE-conjugated Goat Anti-Mouse IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

Anti-Human CD58 (TS2/9) Mouse IgG2a Recombinant Antibody

CD58 Uni-rAbTM Recombinant Antibody for FC
Cat No. 65597-1-MR
CloneNo. TS2/9

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Synonyms

Surface glycoprotein LFA 3, Lymphocyte function-associated antigen 3, LFA3, LFA 3, CD58 molecule

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 647
Size: 

-/ -

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经过测试的应用

Positive FC detected inhuman peripheral blood leukocytes

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65597-1-MR targets CD58 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Human cytolytic T cells 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Full Name CD58 molecule
Synonyms Surface glycoprotein LFA 3, Lymphocyte function-associated antigen 3, LFA3, LFA 3, CD58 molecule
Calculated Molecular Weight 28 kDa
GenBank Accession NumberBC005930
Gene Symbol CD58
Gene ID (NCBI) 965
RRIDAB_3670345
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD58, also known as lymphocyte function-associated antigen 3 (LFA-3), is a heavily glycosylated protein of 55-70 kDa that is expressed on a broad range of hematopoietic and non-hematopoietic cells. CD58 is involved in immune recognition of tumor cells via binding of the CD2 receptor expressed on cytotoxic T cells.

实验方案

Product Specific Protocols
FC protocol for CD58 antibody 65597-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols