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验证数据展示

  • FC experiment of human peripheral blood leukocyte using CL647-65597

    FC experiment of human peripheral blood leukocyte using CL647-65597

    1x10^6 human peripheral blood leukocytes were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD58 (TS2/9) Mouse IgG2a RecAb (CL647-65597, Clone: TS2/9) or CoraLite® Plus 647 Mouse IgG2a Isotype Control (C1.18.4) (CL647-65208, Clone: C1.18.4). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human peripheral blood leukocyte using CL647-65597

    FC experiment of human peripheral blood leukocyte using CL647-65597

    1x10^6 human peripheral blood leukocytes were surface stained with 5 ul CoraLite® Plus 647 Anti-Human CD58 (TS2/9) Mouse IgG2a RecAb (CL647-65597, Clone:TS2/9) (red) or CoraLite® Plus 647 Mouse IgG2a Isotype Control (C1.18.4) (CL647-65208, Clone: C1.18.4) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

CoraLite® Plus 647 Anti-Human CD58 (TS2/9) Mouse IgG2a Recombinant Antibody

CD58 Uni-rAbTM Recombinant Antibody for FC
Cat No. CL647-65597

产品说明书

CloneNo. TS2/9

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

FC

别名

Surface glycoprotein LFA 3, Lymphocyte function-associated antigen 3, LFA3, LFA 3, CD58 molecule

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive FC detected inhuman peripheral blood leukocytes
For other applications, we recommend the unconjugated version of this antibody, 65597-1-MR

推荐稀释比

应用推荐稀释比
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-65597 targets CD58 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 Human cytolytic T cells 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Recombinant
产品类型 Antibody
全称 CD58 molecule
别名 Surface glycoprotein LFA 3, Lymphocyte function-associated antigen 3, LFA3, LFA 3, CD58 molecule
计算分子量 28 kDa
GenBank蛋白编号BC005930
基因名称 CD58
Gene ID (NCBI) 965
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Protein A purification
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD58, also known as lymphocyte function-associated antigen 3 (LFA-3), is a heavily glycosylated protein of 55-70 kDa that is expressed on a broad range of hematopoietic and non-hematopoietic cells. CD58 is involved in immune recognition of tumor cells via binding of the CD2 receptor expressed on cytotoxic T cells.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 CD58 antibody CL647-65597Download protocol
Standard Protocols
Click here to view our Standard Protocols