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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 68222-1-Ig (same clone as 68222-1-PBS)

    WB analysis using 68222-1-Ig (same clone as 68222-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68222-1-Ig (CD59 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 68222-1-Ig (same clone as 68222-1-PBS)

    IHC staining of human placenta using 68222-1-Ig (same clone as 68222-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68222-1-Ig (CD59 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD59 antibody included
  • IF Staining of human placenta using 68222-1-Ig (same clone as 68222-1-PBS)

    IF Staining of human placenta using 68222-1-Ig (same clone as 68222-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CD59 antibody (68222-1-Ig, Clone: 1E3A1 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 68222-1-Ig (same clone as 68222-1-PBS)

    IHC staining of human placenta using 68222-1-Ig (same clone as 68222-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68222-1-Ig (CD59 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD59 antibody included
  • IF Staining of human placenta using 68222-1-Ig (same clone as 68222-1-PBS)

    IF Staining of human placenta using 68222-1-Ig (same clone as 68222-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CD59 antibody (68222-1-Ig, Clone: 1E3A1 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

  • WB analysis using 68222-1-Ig (same clone as 68222-1-PBS)

    WB analysis using 68222-1-Ig (same clone as 68222-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68222-1-Ig (CD59 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

  • IF Staining of BxPC-3 using 68222-1-Ig (same clone as 68222-1-PBS)

    IF Staining of BxPC-3 using 68222-1-Ig (same clone as 68222-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed BxPC-3 cells using CD59 antibody (68222-1-Ig, Clone: 1E3A1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68222-1-PBS in a different storage buffer formulation.

CD59 Monoclonal antibody, PBS Only

CD59 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 68222-1-PBS

产品说明书

CloneNo. 1E3A1

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, IHC, IF/ICC, IF-P, ELISA

别名

HRF-20, CD59 glycoprotein, CD59 antigen, CD 59, 20 kDa homologous restriction factor

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CD59 Ready-To-Use IHC Kit. CD59 primary antibody included.

产品信息

68222-1-PBS targets CD59 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IF-P, ELISA Application Description
经测试反应性 human
免疫原 CD59 fusion protein Ag32834 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 CD59 molecule, complement regulatory protein
别名 HRF-20, CD59 glycoprotein, CD59 antigen, CD 59, 20 kDa homologous restriction factor
计算分子量 19 kDa
观测分子量18-25 kDa
GenBank蛋白编号BC001506
基因名称 CD59
Gene ID (NCBI) 966
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP13987
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD59, also named as MIC11, MIN1, MIN2, MIN3, MSK21, MIRL, MACIF, HRF20 and 1F5 antigen, is a cell surface molecule glycoprotein with MW 18-25 kDa. It acts as a determinant of proximal-distal cell identity. CD59 acts by binding to the C8 and/or C9 complements of the assembling membrane attack complex (MAC), thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. It is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.