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CD59 Monoclonal antibody

CD59 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1E3A1

Cat no : 68222-1-Ig

Print datasheet

Synonyms

HRF-20, CD59 glycoprotein, CD59 antigen, CD 59, 20 kDa homologous restriction factor

验证数据展示

  • WB analysis using 68222-1-Ig

    WB analysis using 68222-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68222-1-Ig (CD59 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 68222-1-Ig

    IHC staining of human placenta using 68222-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68222-1-Ig (CD59 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD59 antibody included
  • IF Staining of human placenta using 68222-1-Ig

    IF Staining of human placenta using 68222-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CD59 antibody (68222-1-Ig, Clone: 1E3A1 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L).

  • IHC staining of human placenta using 68222-1-Ig

    IHC staining of human placenta using 68222-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68222-1-Ig (CD59 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD59 antibody included
  • IF Staining of human placenta using 68222-1-Ig

    IF Staining of human placenta using 68222-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CD59 antibody (68222-1-Ig, Clone: 1E3A1 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L).

  • WB analysis using 68222-1-Ig

    WB analysis using 68222-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68222-1-Ig (CD59 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of BxPC-3 using 68222-1-Ig

    IF Staining of BxPC-3 using 68222-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed BxPC-3 cells using CD59 antibody (68222-1-Ig, Clone: 1E3A1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inhuman colostrum, A549 cells, HeLa cells, MCF-7 cells, HepG2 cells, K-562 cells, Jurkat cells, human placenta tissue
Positive IHC detected inhuman placenta tissue, human lung cancer tissue, human urothelial carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman placenta tissue
Positive IF/ICC detected inBxPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy CD59 Ready-To-Use IHC Kit. CD59 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68222-1-Ig targets CD59 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IF-P, ELISA Application Description
Tested Reactivity human
Immunogen CD59 fusion protein Ag32834 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name CD59 molecule, complement regulatory protein
Synonyms HRF-20, CD59 glycoprotein, CD59 antigen, CD 59, 20 kDa homologous restriction factor
Calculated Molecular Weight 19 kDa
Observed Molecular Weight18-25 kDa
GenBank Accession NumberBC001506
Gene Symbol CD59
Gene ID (NCBI) 966
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP13987
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD59, also named as MIC11, MIN1, MIN2, MIN3, MSK21, MIRL, MACIF, HRF20 and 1F5 antigen, is a cell surface molecule glycoprotein with MW 18-25 kDa. It acts as a determinant of proximal-distal cell identity. CD59 acts by binding to the C8 and/or C9 complements of the assembling membrane attack complex (MAC), thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. It is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.

实验方案

Product Specific Protocols
WB protocol for CD59 antibody 68222-1-IgDownload protocol
IHC protocol for CD59 antibody 68222-1-IgDownload protocol
IF protocol for CD59 antibody 68222-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols