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验证数据展示

  • WB analysis using 68222-1-Ig

    WB analysis using 68222-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68222-1-Ig (CD59 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 68222-1-Ig

    IHC staining of human placenta using 68222-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68222-1-Ig (CD59 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD59 antibody included
  • IF Staining of human placenta using 68222-1-Ig

    IF Staining of human placenta using 68222-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CD59 antibody (68222-1-Ig, Clone: 1E3A1 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L).

  • IHC staining of human placenta using 68222-1-Ig

    IHC staining of human placenta using 68222-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68222-1-Ig (CD59 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD59 antibody included
  • IF Staining of human placenta using 68222-1-Ig

    IF Staining of human placenta using 68222-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CD59 antibody (68222-1-Ig, Clone: 1E3A1 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L).

  • WB analysis using 68222-1-Ig

    WB analysis using 68222-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68222-1-Ig (CD59 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of BxPC-3 using 68222-1-Ig

    IF Staining of BxPC-3 using 68222-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed BxPC-3 cells using CD59 antibody (68222-1-Ig, Clone: 1E3A1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

CD59 Monoclonal antibody

CD59 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 68222-1-Ig

产品说明书

CloneNo. 1E3A1

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, IHC, IF/ICC, IF-P, ELISA

别名

HRF-20, CD59 glycoprotein, CD59 antigen, CD 59, 20 kDa homologous restriction factor

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman colostrum, A549 cells, HeLa cells, MCF-7 cells, HepG2 cells, K-562 cells, Jurkat cells, human placenta tissue
Positive IHC detected inhuman placenta tissue, human lung cancer tissue, human urothelial carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman placenta tissue
Positive IF/ICC detected inBxPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy CD59 Ready-To-Use IHC Kit. CD59 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68222-1-Ig targets CD59 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IF-P, ELISA Application Description
经测试反应性 human
免疫原 CD59 fusion protein Ag32834 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 CD59 molecule, complement regulatory protein
别名 HRF-20, CD59 glycoprotein, CD59 antigen, CD 59, 20 kDa homologous restriction factor
计算分子量 19 kDa
观测分子量18-25 kDa
GenBank蛋白编号BC001506
基因名称 CD59
Gene ID (NCBI) 966
RRIDAB_2935310
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP13987
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD59, also named as MIC11, MIN1, MIN2, MIN3, MSK21, MIRL, MACIF, HRF20 and 1F5 antigen, is a cell surface molecule glycoprotein with MW 18-25 kDa. It acts as a determinant of proximal-distal cell identity. CD59 acts by binding to the C8 and/or C9 complements of the assembling membrane attack complex (MAC), thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. It is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.

实验方案

Product Specific Protocols
WB protocol for CD59 antibody 68222-1-IgDownload protocol
IHC protocol for CD59 antibody 68222-1-IgDownload protocol
IF protocol for CD59 antibody 68222-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols