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验证数据展示

  • IF Staining of human placenta using CL488-68222

    IF Staining of human placenta using CL488-68222

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CoraLite® Plus 488 CD59 antibody (CL488-68222, Clone: 1E3A1 ) at dilution of 1:200.

  • IF Staining of BxPC-3 using CL488-68222

    IF Staining of BxPC-3 using CL488-68222

    Immunofluorescent analysis of (-20°C Ethanol) fixed BxPC-3 cells using CoraLite® Plus 488 CD59 antibody (CL488-68222, Clone: 1E3A1 ) at dilution of 1:200.

  • IF Staining of human placenta using CL488-68222

    IF Staining of human placenta using CL488-68222

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CoraLite® Plus 488 CD59 antibody (CL488-68222, Clone: 1E3A1 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated CD59 Monoclonal antibody

CD59 Monoclonal Antibody for IF/ICC, IF-P
Cat No. CL488-68222

Print datasheet

CloneNo. 1E3A1

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC, IF-P

Synonyms

HRF-20, CD59 glycoprotein, CD59 antigen, CD 59, 20 kDa homologous restriction factor

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman placenta tissue
Positive IF/ICC detected inBxPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy CD59 Ready-To-Use IHC Kit. CD59 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68222-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68222 targets CD59 in IF/ICC, IF-P applications and shows reactivity with human samples.

Tested Applications IF/ICC, IF-P Application Description
Tested Reactivity human
Immunogen CD59 fusion protein Ag32834 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name CD59 molecule, complement regulatory protein
Synonyms HRF-20, CD59 glycoprotein, CD59 antigen, CD 59, 20 kDa homologous restriction factor
Calculated Molecular Weight 19 kDa
Observed Molecular Weight18-25 kDa
GenBank Accession NumberBC001506
Gene Symbol CD59
Gene ID (NCBI) 966
RRIDAB_3084446
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP13987
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD59, also named as MIC11, MIN1, MIN2, MIN3, MSK21, MIRL, MACIF, HRF20 and 1F5 antigen, is a cell surface molecule glycoprotein with MW 18-25 kDa. It acts as a determinant of proximal-distal cell identity. CD59 acts by binding to the C8 and/or C9 complements of the assembling membrane attack complex (MAC), thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. It is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CD59 antibody CL488-68222Download protocol
Standard Protocols
Click here to view our Standard Protocols