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验证数据展示

  • IF Staining of human placenta using CL488-68222

    IF Staining of human placenta using CL488-68222

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CoraLite® Plus 488 CD59 antibody (CL488-68222, Clone: 1E3A1 ) at dilution of 1:200.

  • IF Staining of BxPC-3 using CL488-68222

    IF Staining of BxPC-3 using CL488-68222

    Immunofluorescent analysis of (-20°C Ethanol) fixed BxPC-3 cells using CoraLite® Plus 488 CD59 antibody (CL488-68222, Clone: 1E3A1 ) at dilution of 1:200.

  • IF Staining of human placenta using CL488-68222

    IF Staining of human placenta using CL488-68222

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CoraLite® Plus 488 CD59 antibody (CL488-68222, Clone: 1E3A1 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated CD59 Monoclonal antibody

CD59 Monoclonal Antibody for IF/ICC, IF-P
Cat No. CL488-68222

产品说明书

CloneNo. 1E3A1

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

IF/ICC, IF-P

别名

HRF-20, CD59 glycoprotein, CD59 antigen, CD 59, 20 kDa homologous restriction factor

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman placenta tissue
Positive IF/ICC detected inBxPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy CD59 Ready-To-Use IHC Kit. CD59 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68222-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68222 targets CD59 in IF/ICC, IF-P applications and shows reactivity with human samples.

经测试应用 IF/ICC, IF-P Application Description
经测试反应性 human
免疫原 CD59 fusion protein Ag32834 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 CD59 molecule, complement regulatory protein
别名 HRF-20, CD59 glycoprotein, CD59 antigen, CD 59, 20 kDa homologous restriction factor
计算分子量 19 kDa
观测分子量18-25 kDa
GenBank蛋白编号BC001506
基因名称 CD59
Gene ID (NCBI) 966
RRIDAB_3084446
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP13987
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD59, also named as MIC11, MIN1, MIN2, MIN3, MSK21, MIRL, MACIF, HRF20 and 1F5 antigen, is a cell surface molecule glycoprotein with MW 18-25 kDa. It acts as a determinant of proximal-distal cell identity. CD59 acts by binding to the C8 and/or C9 complements of the assembling membrane attack complex (MAC), thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. It is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CD59 antibody CL488-68222Download protocol
Standard Protocols
Click here to view our Standard Protocols