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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84447-1-RR (same clone as 84447-1-PBS)

    WB analysis using 84447-1-RR (same clone as 84447-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84447-1-RR (CD59 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84447-1-PBS in a different storage buffer formulation.

  • IF Staining of BxPC-3 using 84447-1-RR (same clone as 84447-1-PBS)

    IF Staining of BxPC-3 using 84447-1-RR (same clone as 84447-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed BxPC-3 cells using CD59 antibody (84447-1-RR, Clone: 241310G8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84447-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84447-1-RR

    Affinity and Kinetic Characterization of 84447-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84447-1-RR against Human CD59 were performed. The affinity constant is 1.59 nM.

CD59 Recombinant antibody, PBS Only

CD59 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 84447-1-PBS

产品说明书

CloneNo. 241310G8

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

HRF-20, CD59 glycoprotein, CD59 antigen, CD 59, 241310G8

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CD59 Ready-To-Use IHC Kit. CD59 primary antibody included.

产品信息

84447-1-PBS targets CD59 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 Recombinant Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD59 molecule, complement regulatory protein
别名 HRF-20, CD59 glycoprotein, CD59 antigen, CD 59, 241310G8
计算分子量 19 kDa
观测分子量18-20 kDa
GenBank蛋白编号BC001506
基因名称 CD59
Gene ID (NCBI) 966
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP13987
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD59, also named as MIC11, MIN1, MIN2, MIN3, MSK21, MIRL, MACIF, HRF20 and 1F5 antigen, is a cell surface molecule glycoprotein with MW 18-25 kDa. It acts as a determinant of proximal-distal cell identity. CD59 acts by binding to the C8 and/or C9 complements of the assembling membrane attack complex (MAC), thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. It is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.