验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HUVEC cells, BxPC-3 cells |
| Positive IF/ICC detected in | BxPC-3 cells |
Planning an IHC experiment? We recommend our IHCeasy CD59 Ready-To-Use IHC Kit. CD59 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84447-1-RR targets CD59 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | Recombinant Protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | CD59 molecule, complement regulatory protein |
| 别名 | HRF-20, CD59 glycoprotein, CD59 antigen, CD 59, 241310G8 |
| 计算分子量 | 19 kDa |
| 观测分子量 | 18-20 kDa |
| GenBank蛋白编号 | BC001506 |
| 基因名称 | CD59 |
| Gene ID (NCBI) | 966 |
| RRID | AB_3671973 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | P13987 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD59, also named as MIC11, MIN1, MIN2, MIN3, MSK21, MIRL, MACIF, HRF20 and 1F5 antigen, is a cell surface molecule glycoprotein with MW 18-25 kDa. It acts as a determinant of proximal-distal cell identity. CD59 acts by binding to the C8 and/or C9 complements of the assembling membrane attack complex (MAC), thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. It is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CD59 antibody 84447-1-RR | Download protocol |
| IF protocol for CD59 antibody 84447-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |