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Anti-Human CD59 Rabbit Recombinant Antibody

CD59 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241311A4

Cat no : 98099-1-RR

Print datasheet

Synonyms

HRF-20, CD59 glycoprotein, CD59 antigen, CD 59, 241311A4

验证数据展示

  • FC experiment of human PBMCs using 98099-1-RR

    FC experiment of human PBMCs using 98099-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD59 Rabbit Recombinant Antibody (98099-1-RR, Clone:241311A4) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG, Cells were then stained with APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human PBMCs using 98099-1-RR

    FC experiment of human PBMCs using 98099-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD59 Rabbit Recombinant Antibody (98099-1-RR, Clone:241311A4) or Isotype Control and PE-conjugated Goat Anti-Rabbit IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Affinity and Kinetic Characterization of 98099-1-RR

    Affinity and Kinetic Characterization of 98099-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98099-1-RR against Human CD59 were performed. The affinity constant is 0.888 nM.

经过测试的应用

Positive FC detected inFC receptor blocked human PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD59 Ready-To-Use IHC Kit. CD59 primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98099-1-RR targets CD59 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Recombinant Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD59 molecule, complement regulatory protein
Synonyms HRF-20, CD59 glycoprotein, CD59 antigen, CD 59, 241311A4
Calculated Molecular Weight 19 kDa
GenBank Accession NumberBC001506
Gene Symbol CD59
Gene ID (NCBI) 966
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP13987
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

背景介绍

CD59, also named as MIC11, MIN1, MIN2, MIN3, MSK21, MIRL, MACIF, HRF20 and 1F5 antigen, is a cell surface molecule glycoprotein with MW 18-25 kDa. It acts as a determinant of proximal-distal cell identity. CD59 acts by binding to the C8 and/or C9 complements of the assembling membrane attack complex (MAC), thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. It is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase.

实验方案

Product Specific Protocols
FC protocol for CD59 antibody 98099-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols