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CD5L Polyclonal antibody

CD5L Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17224-1-AP

Print datasheet

Synonyms

hAIM, CT-2, CD5 antigen-like, Apoptosis inhibitor expressed by macrophages, API6

验证数据展示

  • WB analysis of human plasma using 17224-1-AP

    WB analysis of human plasma using 17224-1-AP

    human plasma was subjected to SDS PAGE followed by western blot with 17224-1-AP (CD5L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 17224-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 17224-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 17224-1-AP (CD5L antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 17224-1-AP

    IF Staining of HepG2 using 17224-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CD5L antibody (17224-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inhuman plasma
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17224-1-AP targets CD5L in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen CD5L fusion protein Ag11062 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CD5 molecule-like
Synonyms hAIM, CT-2, CD5 antigen-like, Apoptosis inhibitor expressed by macrophages, API6
Calculated Molecular Weight 347 aa, 38 kDa
Observed Molecular Weight40 kDa
GenBank Accession NumberBC033586
Gene Symbol CD5L
Gene ID (NCBI) 922
RRIDAB_3085517
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43866
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD5 molecule-like (CD5L), also known as apoptosis inhibitor expressed by macrophages (AIM), SP-alpha, apoptosis inhibitor 6 (API6), is a member of the scavenger receptor cysteine-rich (SRCR) family of proteins (PMID: 9045627; 9892623). CD5L is a soluble protein mainly secreted mostly by macrophages in lymphoid and inflamed tissues (PMID: 15009805). It contains three SRCR domains, each approximately 100 amino acids in length with sialic acid content at a potential region of O-linked glycosylation between SRCR domains 1 and 2. CD5L is involved in different processes such as metabolism, infection and autophagy (PMID: 33920819).

实验方案

Product Specific Protocols
WB protocol for CD5L antibody 17224-1-APDownload protocol
IHC protocol for CD5L antibody 17224-1-APDownload protocol
IF protocol for CD5L antibody 17224-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols