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CD6 Recombinant antibody

CD6 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241965D11

Cat no : 84508-5-RR

Print datasheet

Synonyms

TP120, T-cell differentiation antigen CD6, T12, Soluble CD6, CD6 molecule

验证数据展示

  • WB analysis of Jurkat using 84508-5-RR

    WB analysis of Jurkat using 84508-5-RR

    Jurkat cells were subjected to SDS PAGE followed by western blot with 84508-5-RR (CD6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 84508-5-RR

    IHC staining of human tonsillitis using 84508-5-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84508-5-RR (CD6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 84508-5-RR

    IHC staining of human tonsillitis using 84508-5-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84508-5-RR (CD6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inJurkat cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84508-5-RR targets CD6 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen FusionProtein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD6 molecule
Synonyms TP120, T-cell differentiation antigen CD6, T12, Soluble CD6, CD6 molecule
Observed Molecular Weight140 kDa
GenBank Accession NumberBC033755
Gene Symbol CD6
Gene ID (NCBI) 923
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP30203
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD6 is a type I transmembrane glycoprotein that belongs to the scavenger receptor cysteine-rich superfamily (PMID: 21880988). It is composed of an extracellular region, which consists of three tandem SRCR domains, a transmembrane region, and a cytoplasmic tail devoid of intrinsic catalytic activity but harboring several phosphorylatable residues suitable for intracellular signal transduction (PMID: 38139340; 23711376). CD6 is expressed by T cells, medullary thymocytes, a subset of B cells and NK cells, and in some cells of the brain (PMID: 1919444; 21178331). CD6 plays a role in lymphocyte activation, proliferation, and survival processes via interaction with its endogenous ligands.

实验方案

Product Specific Protocols
WB protocol for CD6 antibody 84508-5-RRDownload protocol
IHC protocol for CD6 antibody 84508-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols