CoraLite® Plus 405 Anti-Mouse CD62L (MEL14)
CD62L Monoclonal Antibody for FC
Host / Isotype
Rat / IgG2a, kappa
Reactivity
Mouse
Applications
FC
Conjugate
CoraLite® Plus 405 Fluorescent Dye
CloneNo.
MEL14
验证数据展示
经过测试的应用
| Positive FC detected in | mouse splenocytes |
For other applications, we recommend the unconjugated version of this antibody, 65219-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL405-65219 targets CD62L in FC applications and shows reactivity with Mouse samples.
| Tested Applications | FC Application Description |
| Tested Reactivity | Mouse |
| Immunogen | C3H/eb mouse B lymphoma 38C-13 种属同源性预测 |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | selectin, lymphocyte |
| Synonyms | AI528707, CD62L, L selectin, LECAM 1, Lnhr, Ly 22, Ly m22, Lyam 1, Lyam1, selectin, lymphocyte, Sell |
| GenBank Accession Number | BC052681 |
| Gene Symbol | CD62L |
| Gene ID (NCBI) | 20343 |
| Conjugate | CoraLite® Plus 405 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 399 nm / 422 nm |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | P18337 |
| Storage Buffer | PBS with 0.09% sodium azide. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD62L, also known as L-selectin or SELL, is a member of the selectin family of adhesion molecules that also include CD62E (E-selectin) and CD62P (P-selectin) (PMID: 2663882, 2473156, 1382078). CD62L is a highly glycosylated protein of 95-105 kDa on neutrophils and 74 kDa on lymphocytes (PMID: 1382078; 1694883, 1695155). CD62L is expressed on the surface of most leukocytes, including lymphocytes, neutrophils, monocytes, eosinophils, hematopoietic progenitor cells, and immature thymocytes (PMID: 1694883, 1688580). It mediates the binding of lymphocytes to high endothelial venules (HEV) of peripheral lymph nodes through interactions with a constitutively expressed ligand, and is also involved in lymphocyte, neutrophil, and monocyte attachment to endothelium at sites of inflammation (PMID: 1382078).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 405 CD62L antibody CL405-65219 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
