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Anti-Human CD62P (AK4)

CD62P Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human, Non-human primates

Applications

FC

Conjugate

Unconjugated

CloneNo.

AK4

Cat no : 65164-1-Ig

Print datasheet

Synonyms

AK4, CD62, CD62P, FLJ45155, GMP 140, GMP140, GMRP, Granule membrane protein 140, GRMP, LECAM3, P selectin, PADGEM, PSEL, SELP

验证数据展示

  • FC experiment of human peripheral blood platelets using 65164-1-Ig

    FC experiment of human peripheral blood platelets using 65164-1-Ig

    1X10^6 thrombin-activated human peripheral blood platelets were surface co-stained with Anti-Human CD41a labeled with FlexAble CL488 and 0.2 ug Anti-Human CD62P (65164-1-Ig, Clone:AK4) labeled with FlexAble CL647. Cells were not fixed.

  • FC experiment of human peripheral blood platelets using 65164-1-Ig

    FC experiment of human peripheral blood platelets using 65164-1-Ig

    1X10^6 thrombin-activated human peripheral blood platelets were surface stained with 0.2 ug Anti-Human CD62P (65164-1-Ig, Clone:AK4) labeled with FlexAble CL647. Cells were not fixed.

经过测试的应用

Positive FC detected inhuman peripheral blood platelets
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite® Plus 555 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65164-1-Ig targets CD62P in FC applications and shows reactivity with Human, Non-human primates samples.

Tested Applications FC Application Description
Tested Reactivity Human, Non-human primates
Immunogen Human platelets 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name selectin P (granule membrane protein 140kDa, antigen CD62)
Synonyms AK4, CD62, CD62P, FLJ45155, GMP 140, GMP140, GMRP, Granule membrane protein 140, GRMP, LECAM3, P selectin, PADGEM, PSEL, SELP
Calculated Molecular Weight 830 aa, 91 kDa
GenBank Accession NumberBC028067
Gene Symbol CD62P
Gene ID (NCBI) 6403
RRIDAB_2918443
Conjugate Unconjugated
Form Liquid
Purification MethodPurified by protein-A affinity chromatography
UNIPROT IDP16109
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

P-selectin, also known as GMP140 or CD62P, is a transmembrane glycoprotein that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is an adhesion molecule involved in the pathogenesis of inflammation, thrombosis, and oncogenesis. P-selectin is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.

实验方案

Product Specific Protocols
FC protocol for CD62P antibody 65164-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols