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验证数据展示

  • FC experiment of human peripheral blood platelets using CL594-65164

    FC experiment of human peripheral blood platelets using CL594-65164

    100ul thrombin-activated human platelet-rich plasma (diluted 1:2 in PBS) was surface stained with CL405 Anti-Human CD41a (CL405-65173) and 5 ul CoraLite® Plus 594 Anti-Human CD62P (CL594-65164, Clone:AK4) or CoraLite®594 Mouse IgG1 Isotype Control (CL594-65124). Cells were not fixed.

  • FC experiment of human peripheral blood platelets using CL594-65164

    FC experiment of human peripheral blood platelets using CL594-65164

    100ul thrombin-activated human platelet-rich plasma (diluted 1:2 in PBS) was surface stained with 5 ul CoraLite® Plus 594 Anti-Human CD62P (CL594-65164, Clone:AK4) or CoraLite®594 Mouse IgG1 Isotype Control (CL594-65124). Cells were not fixed.

CoraLite® Plus 594 Anti-Human CD62P (AK4)

CD62P Monoclonal Antibody for FC
Cat No. CL594-65164

产品说明书

CloneNo. AK4

宿主/亚型

Mouse / IgG1, kappa

种属反应性

human, non-human primates

应用

FC

别名

P-selectin, GMRP, GMP-140, GMP140, GMP 140

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 594
规格: 

-/ -

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经过测试的应用

Positive FC detected inthrombin-activated human peripheral blood platelets
For other applications, we recommend the unconjugated version of this antibody, 65164-1-Ig

推荐稀释比

应用推荐稀释比
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-65164 targets CD62P in FC applications and shows reactivity with human, non-human primates samples.

经测试应用 FC Application Description
经测试反应性 human, non-human primates
免疫原 Human platelets 种属同源性预测
宿主/亚型 Mouse / IgG1, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 selectin P (granule membrane protein 140kDa, antigen CD62)
别名 P-selectin, GMRP, GMP-140, GMP140, GMP 140
计算分子量 830 aa, 91 kDa
GenBank蛋白编号BC028067
基因名称 CD62P
Gene ID (NCBI) 6403
偶联类型 CoraLite® Plus 594 Fluorescent Dye
最大激发/发射波长594 nm / 615 nm
形式 Liquid
纯化方式Purified by protein-A affinity chromatography
UNIPROT IDP16109
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

P-selectin, also known as GMP140 or CD62P, is a transmembrane glycoprotein that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is an adhesion molecule involved in the pathogenesis of inflammation, thrombosis, and oncogenesis. P-selectin is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.

实验方案

Product Specific Protocols
FC protocol for CL Plus 594 CD62P antibody CL594-65164Download protocol
Standard Protocols
Click here to view our Standard Protocols