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CD63 Recombinant antibody

CD63 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241964H1

Cat no : 84507-4-RR

Print datasheet

Synonyms

Lysosome integral membrane protein 1, Lysosomal-associated membrane protein 3, Limp1, CD63 antigen, CD 63

验证数据展示

  • WB analysis using 84507-4-RR

    WB analysis using 84507-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84507-4-RR (CD63 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 84507-4-RR

    IHC staining of human tonsillitis using 84507-4-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84507-4-RR (CD63 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD63 antibody included
  • IF Staining of A431 using 84507-4-RR

    IF Staining of A431 using 84507-4-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CD63 antibody (84507-4-RR, Clone: 241964H1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IHC staining of human tonsillitis using 84507-4-RR

    IHC staining of human tonsillitis using 84507-4-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84507-4-RR (CD63 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD63 antibody included

经过测试的应用

Positive WB detected inU-87 MG cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy CD63 Ready-To-Use IHC Kit. CD63 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84507-4-RR targets CD63 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen FusionProtein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD63 molecule
Synonyms Lysosome integral membrane protein 1, Lysosomal-associated membrane protein 3, Limp1, CD63 antigen, CD 63
Calculated Molecular Weight 26 kDa
Observed Molecular Weight30-60 kDa
GenBank Accession NumberBC002349
Gene Symbol CD63
Gene ID (NCBI) 967
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP08962
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD63 is a 30-60 kDa lysosomal membrane protein that belongs to the tetraspanin family. This protein plays many important roles in immuno-physiological functions. It mediates signal transduction events that regulate cell development, activation, growth, and motility. CD63 is expressed on activated platelets, thus it may function as a blood platelet activation marker. CD63 is a lysosomal membrane glycoprotein translocated to the plasma membrane after platelet activation. The CD63 tetraspanin is highly expressed in the early stages of melanoma and decreases in advanced lesions, suggesting it is a possible suppressor of tumor progression. Deficiency of this protein is associated with Hermansky-Pudlak syndrome.

实验方案

Product Specific Protocols
WB protocol for CD63 antibody 84507-4-RRDownload protocol
IHC protocol for CD63 antibody 84507-4-RRDownload protocol
IF protocol for CD63 antibody 84507-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols