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CD64 Polyclonal antibody, PBS Only

CD64 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 27563-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis using 27563-1-AP (same clone as 27563-1-PBS)

    WB analysis using 27563-1-AP (same clone as 27563-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 27563-1-AP (CD64 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27563-1-PBS in a different storage buffer formulation.

  • IF Staining of THP-1 using 27563-1-AP (same clone as 27563-1-PBS)

    IF Staining of THP-1 using 27563-1-AP (same clone as 27563-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CD64 antibody (27563-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 27563-1-PBS in a different storage buffer formulation.

产品信息

27563-1-PBS targets CD64 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen CD64 fusion protein Ag26727 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Fc fragment of IgG, high affinity Ia, receptor (CD64)
Synonyms
Calculated Molecular Weight 374 aa, 43 kDa
Observed Molecular Weight 65-70 kDa, 43-45 kDa
GenBank Accession NumberBC032634
Gene Symbol FCGR1A
Gene ID (NCBI) 2209
ENSEMBL Gene IDENSG00000150337
RRIDAB_2880909
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP12314
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Fcγ receptor comprise a multigene family of integral membrane glycoproteins that exhibit complex activation or inhibitory effects on cell functions after aggregation by complexed immunoglobulin G (IgG) (PMID: 17005690 ). CD64, also known as FcγRIA, is a high affinity receptor for the Fc region of IgG. It is expressed by monocytes/macrophages, activated neutrophils, dendritic cells, and early myeloid cells (PMID: 23293080; 19642859; 7680917). CD64 functions in both innate and adaptive immune responses. The calculated molecular weight of CD64 is 43 kDa, while the glycosylated CD64 has a higher apparent molecular weight.