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CoraLite® Plus 405 Anti-Human CD64 (10.1) Mouse IgG2a Recombinant Antibody

CD64 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 405 Fluorescent Dye

CloneNo.

10.1

Cat no : CL405-65572

Print datasheet

Synonyms

Fc-gamma RI, FCG1, Fc gamma RIA, Fc gamma RI, CD64A

验证数据展示

  • FC experiment of human PBMCs using CL405-65572

    FC experiment of human PBMCs using CL405-65572

    1x10^6 human PBMCs were surface stained with CoraLite® Plus 647 Anti-Human CD11b and 5 ul CoraLite® Plus 405 Anti-Human CD64 (10.1) Mouse IgG2a Recombinant Antibody (CL405-65572, Clone: 10.1) (right), or CoraLite® Plus 405 Mouse IgG2a Isotype Control (C1.18.4) (CL405-65208, Clone: C1.18.4) (left). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human PBMCs using CL405-65572

    FC experiment of human PBMCs using CL405-65572

    1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 405 Anti-Human CD64 (10.1) Mouse IgG2a Recombinant Antibody (CL405-65572, Clone: 10.1) (red), or CoraLite® Plus 405 Mouse IgG2a Isotype Control (C1.18.4) (CL405-65208, Clone: C1.18.4) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

经过测试的应用

Positive FC detected inhuman PBMCs
For other applications, we recommend the unconjugated version of this antibody, 65572-1-MR

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL405-65572 targets CD64 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Human rheumatoid synovial fluid cells and fibronectin-purified monocytes. 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Full Name Fc fragment of IgG, high affinity Ia, receptor (CD64)
Synonyms Fc-gamma RI, FCG1, Fc gamma RIA, Fc gamma RI, CD64A
Calculated Molecular Weight 374 aa, 43 kDa
GenBank Accession NumberBC032634
Gene Symbol FCGR1A
Gene ID (NCBI) 2209
ENSEMBL Gene IDENSG00000150337
Conjugate CoraLite® Plus 405 Fluorescent Dye
Excitation/Emission Maxima Wavelengths399 nm / 422 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

Fcγ receptor comprise a multigene family of integral membrane glycoproteins that exhibit complex activation or inhibitory effects on cell functions after aggregation by complexed immunoglobulin G (IgG) (PMID: 17005690 ). CD64, also known as FcγRIA, is a high-affinity receptor for the Fc region of IgG. It is expressed by monocytes/macrophages, activated neutrophils, dendritic cells, and early myeloid cells (PMID: 23293080; 19642859; 7680917). CD64 functions in both innate and adaptive immune responses.

实验方案

Product Specific Protocols
FC protocol for CL Plus 405 CD64 antibody CL405-65572Download protocol
Standard Protocols
Click here to view our Standard Protocols