验证数据展示
经过测试的应用
| Positive WB detected in | U-937 cells, RAW 264.7 cells |
Planning an IHC experiment? We recommend our IHCeasy CD68 Ready-To-Use IHC Kit. CD68 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66231-1-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-66231 targets CD68 in WB applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | CD68 fusion protein Ag22815 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | CD68 molecule |
| 别名 | CD68, CD68 molecule, DKFZp686M18236, GP110, Macrosialin, SCARD1 |
| 计算分子量 | 37 kDa |
| 观测分子量 | 70-100 kDa |
| GenBank蛋白编号 | BC015557 |
| 基因名称 | CD68 |
| Gene ID (NCBI) | 968 |
| RRID | AB_3084891 |
| 偶联类型 | CoraLite® Plus 750 Fluorescent Dye |
| 最大激发/发射波长 | 755 nm / 780 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P34810 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 750 CD68 antibody CL750-66231 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |