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验证数据展示

  • FC experiment of human PBMCs using FITC-65593

    FC experiment of human PBMCs using FITC-65593

    1x10^6 human PBMCs were intracellularly stained with APC Anti-Human CD14 and 5 ul FITC Plus Anti-Human CD68 (KP1) Mouse IgG2a RecAb (FITC-65593, Clone: KP1) or FITC Plus Mouse IgG2a Isotype Control (C1.18.4) (FITC-65208, Clone: C1.18.4). Cells were incubated with FC Receptor Block prior to staining. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of human PBMCs using FITC-65593

    FC experiment of human PBMCs using FITC-65593

    1x10^6 human PBMCs were intracellularly stained with 5 ul FITC Plus Anti-Human CD68 (KP1) Mouse IgG2a RecAb (FITC-65593, Clone: KP1) (red) or FITC Plus Mouse IgG2a Isotype Control (C1.18.4) (FITC-65208, Clone: C1.18.4) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

FITC Plus Anti-Human CD68 (KP1) Mouse IgG2a Recombinant Antibody

CD68 Uni-rAbTM Recombinant Antibody for FC (Intra)
Cat No. FITC-65593

Print datasheet

CloneNo. KP1

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC (Intra)

Synonyms

SCARD1, Macrosialin, KP1, GP110, CD68 molecule

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  FITC Plus
Size: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD68 Ready-To-Use IHC Kit. CD68 primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

FITC-65593 targets CD68 in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Full Name CD68 molecule
Synonyms SCARD1, Macrosialin, KP1, GP110, CD68 molecule
Calculated Molecular Weight 37 kDa
GenBank Accession NumberBC015557
Gene Symbol CD68
Gene ID (NCBI) 968
Conjugate FITC Plus Fluorescent Dye
Excitation/Emission Maxima Wavelengths495 nm / 524 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD68 is a type I transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It belongs to the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family and primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. CD68 is also a member of the scavenger receptor family. It may play a role in phagocytic activities of tissue macrophages.

实验方案

Product Specific Protocols
FC protocol for FITC Plus CD68 antibody FITC-65593Download protocol
Standard Protocols
Click here to view our Standard Protocols