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Anti-Mouse CD69 (H1.2F3)

CD69 Monoclonal Antibody for FC

Host / Isotype

Armenian Hamster / IgG

Reactivity

Mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

H1.2F3

Cat no : 65105-1-Ig

Print datasheet

Synonyms

5830438K24Rik, AI452015, AIM, CD69, CD69 antigen, VEA

验证数据展示

  • FC experiment of mouse splenocytes using 65105-1-Ig

    FC experiment of mouse splenocytes using 65105-1-Ig

    1X10^6 Anti-CD3/CD28-stimulated mouse splenocytes were surface stained with 0.5 ug Anti-Mouse CD69 (65105-1-Ig, Clone:H1.2F3) (red), or 0.5 ug Isotype Control. Cells were not fixed.

经过测试的应用

Positive FC detected inAnti-CD3/CD28-stimulated mouse splenocytes

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65105-1-Ig targets CD69 in FC applications and shows reactivity with Mouse samples.

Tested Applications FC Application Description
Tested Reactivity Mouse
Immunogen Mouse dendritic epidermal T cell line Y245 种属同源性预测
Host / Isotype Armenian Hamster / IgG
Class Monoclonal
Type Antibody
Full Name CD69 antigen
Synonyms 5830438K24Rik, AI452015, AIM, CD69, CD69 antigen, VEA
GenBank Accession NumberBC106997
Gene Symbol Cd69
Gene ID (NCBI) 12515
RRIDAB_2918404
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD69, also known as AIM, EA-1, Leu-23, and MLR3, is a type II transmembrane glycoprotein that belongs to the C-type lectin superfamily (PMID: 8340758; 7804122). CD69 is constitutively expressed by mature thymocytes, platelets, several subsets of tissue resident immune cells (including resident memory T cells and gamma delta T cells), and is inducibly expressed by activated T cells, B cells, natural killer (NK) cells, monocytes, neutrophils (PMID: 8100776; 28475283). CD69 has been identified as an early activation marker of lymphocytes and is commonly used as a marker of activated lymphocytes and NK cells (PMID: 28475283; 25759842). It is involved in the regulation of immune responses (PMID: 15745855).

实验方案

Product Specific Protocols
FC protocol for CD69 antibody 65105-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols