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CoraLite® Plus 750 Anti-Mouse CD69 (H1.2F3)

CD69 Monoclonal Antibody for FC

Host / Isotype

Armenian Hamster / IgG

Reactivity

Mouse

Applications

FC

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

H1.2F3

Cat no : CL750-65105

Print datasheet

Synonyms

5830438K24Rik, AI452015, AIM, Cd69, CD69 antigen, VEA

验证数据展示

  • FC experiment of mouse splenocytes using CL750-65105

    FC experiment of mouse splenocytes using CL750-65105

    1X10^6 unstimulated or Anti-CD3/CD28-stimulated mouse splenocytes were surface stained with 0.5 ug CoraLite® Plus 750 Anti-Mouse CD69 (CL750-65105, Clone: H1.2F3). Cells were not fixed.

  • FC experiment of mouse splenocytes using CL750-65105

    FC experiment of mouse splenocytes using CL750-65105

    1X10^6 Anti-CD3/CD28-stimulated mouse splenocytes were surface stained with 0.5 ug CoraLite® Plus 750 Anti-Mouse CD69 (CL750-65105, Clone: H1.2F3) (red), or 0.5 ug Isotype Control (blue). The black dashed line indicates unstimulated mouse splenocytes surface stained with 0.5 ug CoraLite® Plus 750 Anti-Mouse CD69 (CL750-65105, Clone: H1.2F3). Cells were not fixed.

经过测试的应用

Positive FC detected inAnti-CD3/CD28-stimulated mouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65105-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-65105 targets CD69 in FC applications and shows reactivity with Mouse samples.

Tested Applications FC Application Description
Tested Reactivity Mouse
Immunogen Mouse dendritic epidermal T cell line Y245 种属同源性预测
Host / Isotype Armenian Hamster / IgG
Class Monoclonal
Type Antibody
Full Name CD69 antigen
Synonyms 5830438K24Rik, AI452015, AIM, Cd69, CD69 antigen, VEA
GenBank Accession NumberBC106997
Gene Symbol Cd69
Gene ID (NCBI) 12515
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD69, also known as AIM, EA-1, Leu-23, and MLR3, is a type II transmembrane glycoprotein that belongs to the C-type lectin superfamily (PMID: 8340758; 7804122). CD69 is constitutively expressed by mature thymocytes, platelets, several subsets of tissue resident immune cells (including resident memory T cells and gamma delta T cells), and is inducibly expressed by activated T cells, B cells, natural killer (NK) cells, monocytes, neutrophils (PMID: 8100776; 28475283). CD69 has been identified as an early activation marker of lymphocytes and is commonly used as a marker of activated lymphocytes and NK cells (PMID: 28475283; 25759842). It is involved in the regulation of immune responses (PMID: 15745855).

实验方案

Product Specific Protocols
FC protocol for CL Plus 750 CD69 antibody CL750-65105Download protocol
Standard Protocols
Click here to view our Standard Protocols