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PE Anti-Human CD69 Rabbit Recombinant Antibody

CD69 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

241552F1

Cat no : PE-98173

Print datasheet

Synonyms

CD69 molecule, BL-AC/P26, BL AC/P26, AIM, Activation inducer molecule

验证数据展示

  • FC experiment of human PBMCs using PE-98173

    FC experiment of human PBMCs using PE-98173

    1x10^6 PHA treated human PBMCs were surface stained with CoraLite® Plus 647 Anti-Human CD4 and 5 ul PE Anti-Human CD69 Rabbit Recombinant Antibody (PE-98173, Clone:241552F1) or 1x10^6 untreated human PBMCs were surface stained with 5 ul PE Anti-Human CD69 Rabbit Recombinant Antibody (PE-98173, Clone:241552F1). Cells were not fixed.

  • FC experiment of human PBMCs using PE-98173

    FC experiment of human PBMCs using PE-98173

    1x10^6 PHA treated human PBMCs were surface stained with 5 ul PE Anti-Human CD69 Rabbit Recombinant Antibody (PE-98173, Clone:241552F1)(red) or PE Rabbit IgG Isotype Control Recombinant Antibody (PE-98136, Clone: 240953C9) (blue). 1x10^6 untreated human PBMCs were surface stained with 5 ul PE Anti-Human CD69 Rabbit Recombinant Antibody (PE-98173, Clone: 241552F1) (black, dashed). Cells were not fixed.

经过测试的应用

Positive FC detected inPHA treated human PBMCs

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

PE-98173 targets CD69 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD69 molecule
Synonyms CD69 molecule, BL-AC/P26, BL AC/P26, AIM, Activation inducer molecule
Calculated Molecular Weight23 kDa
GenBank Accession NumberNM_001781.2
Gene Symbol CD69
Gene ID (NCBI) 969
Conjugate PE Fluorescent Dye
Excitation/Emission Maxima Wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ07108
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD69, also known as AIM, EA-1, Leu-23, and MLR3, is a type II transmembrane glycoprotein that belongs to the C-type lectin superfamily (PMID: 8340758; 7804122). CD69 is constitutively expressed by mature thymocytes, platelets, several subsets of tissue resident immune cells (including resident memory T cells and gamma delta T cells), and is inducibly expressed by activated T cells, B cells, natural killer (NK) cells, monocytes, neutrophils (PMID: 8100776; 28475283). CD69 has been identified as an early activation marker of lymphocytes and is commonly used as a marker of activated lymphocytes and NK cells (PMID: 28475283; 25759842). It is involved in the regulation of immune responses (PMID: 15745855).

实验方案

Product Specific Protocols
FC protocol for PE CD69 antibody PE-98173Download protocol
Standard Protocols
Click here to view our Standard Protocols