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验证数据展示

  • WB analysis of Jurkat using 60209-2-Ig

    WB analysis of Jurkat using 60209-2-Ig

    Untreated and PNGase F-treated lysates of Jurkat cells were subjected to SDS PAGE followed by western blot with 60209-2-Ig (CD7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

  • WB analysis using 60209-2-Ig

    WB analysis using 60209-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60209-2-Ig (CD7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 60209-2-Ig

    IHC staining of human tonsillitis using 60209-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60209-2-Ig (CD7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD7 antibody included
  • IHC staining of human tonsillitis using 60209-2-Ig

    IHC staining of human tonsillitis using 60209-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60209-2-Ig (CD7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD7 antibody included
  • IHC staining of human tonsillitis using 60209-2-Ig

    IHC staining of human tonsillitis using 60209-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60209-2-Ig (CD7 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD7 antibody included

CD7 Monoclonal antibody

CD7 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 60209-2-Ig

产品说明书

CloneNo. 2A4E6

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

T cell leukemia antigen, T cell antigen CD7, LEU-9, GP40, CD7 molecule

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, HepG2 cells, HuH-7 cells, MOLT-4 cells
Positive IHC detected inhuman tonsillitis tissue, human appendicitis tissue, human lymphoma tissue, mouse thymus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CD7 Ready-To-Use IHC Kit. CD7 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60209-2-Ig targets CD7 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 CD7 fusion protein Ag1805 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 CD7 molecule
别名 T cell leukemia antigen, T cell antigen CD7, LEU-9, GP40, CD7 molecule
计算分子量 240 aa, 25 kDa
观测分子量 34-36 kDa
GenBank蛋白编号BC009293
基因名称 CD7
Gene ID (NCBI) 924
ENSEMBL Gene IDENSG00000173762
RRIDAB_2881355
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP09564
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD7 is a type I transmembrane glycoprotein expressed on thymocytes and mature T cells. It is the most reliable clinical marker of T-cell acute lymphocytic leukemia.

实验方案

Product Specific Protocols
WB protocol for CD7 antibody 60209-2-IgDownload protocol
IHC protocol for CD7 antibody 60209-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols