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CD7 Monoclonal antibody

CD7 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2A4E6

Cat no : 60209-2-Ig

Print datasheet

Synonyms

T cell leukemia antigen, T cell antigen CD7, LEU-9, GP40, CD7 molecule

验证数据展示

  • WB analysis of Jurkat using 60209-2-Ig

    WB analysis of Jurkat using 60209-2-Ig

    Untreated and PNGase F-treated lysates of Jurkat cells were subjected to SDS PAGE followed by western blot with 60209-2-Ig (CD7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

  • WB analysis using 60209-2-Ig

    WB analysis using 60209-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 60209-2-Ig (CD7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 60209-2-Ig

    IHC staining of human tonsillitis using 60209-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60209-2-Ig (CD7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD7 antibody included
  • IHC staining of human tonsillitis using 60209-2-Ig

    IHC staining of human tonsillitis using 60209-2-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60209-2-Ig (CD7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD7 antibody included

经过测试的应用

Positive WB detected inJurkat cells, HepG2 cells, HuH-7 cells, MOLT-4 cells
Positive IHC detected inhuman tonsillitis tissue, human lymphoma tissue, mouse thymus tissue, human appendicitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CD7 Ready-To-Use IHC Kit. CD7 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60209-2-Ig targets CD7 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen CD7 fusion protein Ag1805 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name CD7 molecule
Synonyms T cell leukemia antigen, T cell antigen CD7, LEU-9, GP40, CD7 molecule
Calculated Molecular Weight 240 aa, 25 kDa
Observed Molecular Weight 34-36 kDa
GenBank Accession NumberBC009293
Gene Symbol CD7
Gene ID (NCBI) 924
ENSEMBL Gene IDENSG00000173762
RRIDAB_2881355
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP09564
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD7 is a type I transmembrane glycoprotein expressed on thymocytes and mature T cells. It is the most reliable clinical marker of T-cell acute lymphocytic leukemia.

实验方案

Product Specific Protocols
WB protocol for CD7 antibody 60209-2-IgDownload protocol
IHC protocol for CD7 antibody 60209-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols