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CD7 Monoclonal antibody, PBS Only

CD7 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A4E6

Cat no : 60209-2-PBS

Print datasheet

Synonyms

T cell leukemia antigen, T cell antigen CD7, LEU-9, GP40, CD7 molecule

验证数据展示

  • WB analysis of Jurkat using 60209-2-Ig (same clone as 60209-2-PBS)

    WB analysis of Jurkat using 60209-2-Ig (same clone as 60209-2-PBS)

    Untreated and PNGase F-treated lysates of Jurkat cells were subjected to SDS PAGE followed by western blot with 60209-2-Ig (CD7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 60209-2-PBS in a different storage buffer formulation.

  • WB analysis using 60209-2-Ig (same clone as 60209-2-PBS)

    WB analysis using 60209-2-Ig (same clone as 60209-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60209-2-Ig (CD7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60209-2-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 60209-2-Ig (same clone as 60209-2-PBS)

    IHC staining of human tonsillitis using 60209-2-Ig (same clone as 60209-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60209-2-Ig (CD7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60209-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD7 antibody included
  • IHC staining of human tonsillitis using 60209-2-Ig (same clone as 60209-2-PBS)

    IHC staining of human tonsillitis using 60209-2-Ig (same clone as 60209-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60209-2-Ig (CD7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60209-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD7 antibody included
Planning an IHC experiment? We recommend our IHCeasy CD7 Ready-To-Use IHC Kit. CD7 primary antibody included.

产品信息

60209-2-PBS targets CD7 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen CD7 fusion protein Ag1805 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name CD7 molecule
Synonyms T cell leukemia antigen, T cell antigen CD7, LEU-9, GP40, CD7 molecule
Calculated Molecular Weight 240 aa, 25 kDa
Observed Molecular Weight 34-36 kDa
GenBank Accession NumberBC009293
Gene Symbol CD7
Gene ID (NCBI) 924
ENSEMBL Gene IDENSG00000173762
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP09564
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD7 is a type I transmembrane glycoprotein expressed on thymocytes and mature T cells. It is the most reliable clinical marker of T-cell acute lymphocytic leukemia.