验证数据展示
经过测试的应用
| Positive FC detected in | U266 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
PE-FcA98098 targets CD70 in FC applications and shows reactivity with human samples.
| 经测试应用 | FC Application Description |
| 经测试反应性 | human |
| 免疫原 | Recombinant Protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | CD70 molecule |
| 别名 | 241325D8, CD27 L, CD27 ligand, CD27L, CD27-L |
| 计算分子量 | 21 kDa |
| GenBank蛋白编号 | BC000725 |
| 基因名称 | CD70 |
| Gene ID (NCBI) | 970 |
| 偶联类型 | PE Fluorescent Dye |
| 最大激发/发射波长 | 496 nm, 565 nm / 578 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3 |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD70 is the membrane-bound ligand of the CD27 receptor, which belongs to the tumor necrosis factor receptor superfamily. CD70 is not expressed in non-lymphoid normal tissue but is overexpressed in tumor cells of various solid cancers, diffuse large B-cell and follicular lymphoma and the malignant cells of Hodgkin lymphoma, Waldenström macroglobulinemia, and multiple myeloma (PMID: 28651374, 20699361 ). CD70/CD27 interactions play an important role in providing co-stimulation during the development of functional lymphocytes; strict control of CD70 expression is required for optimal signaling for immune cell activation (PMID: 21304103).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for PE CD70 antibody PE-FcA98098 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |