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Anti-Human CD71 (OKT9)

CD71 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

OKT9

Cat no : 65235-1-Ig

Print datasheet

Synonyms

CD71, p90, sTfR, T9, TFR, TFR1, TFRC, TR, Transferrin receptor protein 1, TRFR

验证数据展示

  • FC experiment of human PBMCs using 65235-1-Ig

    FC experiment of human PBMCs using 65235-1-Ig

    1X10^6 day 3 PHA treated human PBMCs were surface stained with 0.2 ug Anti-Human CD71 (65235-1-Ig, Clone:OKT9) and CL594-Goat anti-Mouse IgG at dilution 1:1000. Cells were not fixed. Lymphocytes were gated. Blue line indicates unstained stimulated cells. Black dashed line indicates unstimulated stained cells.

经过测试的应用

Positive FC detected inPHA treated human PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD71 Ready-To-Use IHC Kit. CD71 primary antibody included.

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65235-1-Ig targets CD71 in FC applications and shows reactivity with Human samples.

Tested Applications FC Application Description
Tested Reactivity Human
Immunogen Human T-cell acute lymphoblastic leukemia cells 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name transferrin receptor (p90, CD71)
Synonyms CD71, p90, sTfR, T9, TFR, TFR1, TFRC, TR, Transferrin receptor protein 1, TRFR
Calculated Molecular Weight 85 kDa
GenBank Accession NumberBC001188
Gene Symbol CD71
Gene ID (NCBI) 7037
ENSEMBL Gene IDENSG00000072274
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP02786
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex that enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.

实验方案

Product Specific Protocols
FC protocol for CD71 antibody 65235-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols