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验证数据展示

  • FC experiment of human PBMCs using APC-65235

    FC experiment of human PBMCs using APC-65235

    1x10^6 PHA treated (3 day) human PBMCs were surface stained with 5 ul APC Anti-Human CD71 (APC-65235, Clone: OKT9) (red) or APC Mouse IgG1 Isotype Control (APC-65124, Clone: MOPC-21) (blue). 1x10^6 untreated human PBMCs were surface stained with 5 ul APC Anti-Human CD71 (APC-65235, Clone: OKT9) (black dashed). Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using APC-65235

    FC experiment of human PBMCs using APC-65235

    1x10^6 PHA treated (3 day) human PBMCs were surface stained with 5 ul APC Anti-Human CD71 (APC-65235, Clone: OKT9) (right) or APC Mouse IgG1 Isotype Control (APC-65124, Clone: MOPC-21) (left). 1x10^6 untreated human PBMCs were surface stained with 5 ul APC Anti-Human CD71 (APC-65235, Clone: OKT9) (center). Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes were gated.

APC Anti-Human CD71 (OKT9)

CD71 Monoclonal Antibody for FC
Cat No. APC-65235

产品说明书

CloneNo. OKT9

宿主/亚型

Mouse / IgG1, kappa

种属反应性

human

应用

FC

别名

TFR1, TFR, T9, sTfR, p90

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  APC
规格: 

-/ -

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经过测试的应用

Positive FC detected inPHA treated human PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD71 Ready-To-Use IHC Kit. CD71 primary antibody included.

推荐稀释比

应用推荐稀释比
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

APC-65235 targets CD71 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 Human T-cell acute lymphoblastic leukemia cells 种属同源性预测
宿主/亚型 Mouse / IgG1, kappa
抗体类别 Monoclonal
产品类型 Antibody
全称 transferrin receptor (p90, CD71)
别名 TFR1, TFR, T9, sTfR, p90
计算分子量 85 kDa
GenBank蛋白编号BC001188
基因名称 CD71
Gene ID (NCBI) 7037
ENSEMBL Gene IDENSG00000072274
RRIDAB_3672347
偶联类型 APC Fluorescent Dye
最大激发/发射波长650 nm / 660 nm
形式 Liquid
纯化方式N/A
UNIPROT IDP02786
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex that enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.

实验方案

Product Specific Protocols
FC protocol for APC CD71 antibody APC-65235Download protocol
Standard Protocols
Click here to view our Standard Protocols