验证数据展示
经过测试的应用
| Positive WB detected in | PC-12 cells, HeLa cells, HT-1080 cells, Raji cells, K-562 cells |
| Positive IF/ICC detected in | K-562 cells |
Planning an IHC experiment? We recommend our IHCeasy CD71 Ready-To-Use IHC Kit. CD71 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
81793-2-RR targets CD71 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | CD71 fusion protein Ag0123 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | transferrin receptor (p90, CD71) |
| 别名 | TFR1, TFR, T9, sTfR, p90 |
| 计算分子量 | 85 kDa |
| 观测分子量 | 90 kDa |
| GenBank蛋白编号 | BC001188 |
| 基因名称 | CD71 |
| Gene ID (NCBI) | 7037 |
| ENSEMBL Gene ID | ENSG00000072274 |
| RRID | AB_3670505 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P02786 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex that enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CD71 antibody 81793-2-RR | Download protocol |
| IF protocol for CD71 antibody 81793-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |