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CoraLite® Plus 488-conjugated CD71 Recombinant antibody

CD71 Uni-rAbTM Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2P14

Cat no : CL488-81793-2

Print datasheet

Synonyms

TFR1, TFR, T9, sTfR, p90

验证数据展示

  • IF Staining of K-562 using CL488-81793-2

    IF Staining of K-562 using CL488-81793-2

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CoraLite® Plus 488 CD71 antibody (CL488-81793-2, Clone: 2P14 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy CD71 Ready-To-Use IHC Kit. CD71 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 81793-2-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-81793-2 targets CD71 in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen CD71 fusion protein Ag0123 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name transferrin receptor (p90, CD71)
Synonyms TFR1, TFR, T9, sTfR, p90
Calculated Molecular Weight 85 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC001188
Gene Symbol CD71
Gene ID (NCBI) 7037
ENSEMBL Gene IDENSG00000072274
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP02786
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex that enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CD71 antibody CL488-81793-2Download protocol
Standard Protocols
Click here to view our Standard Protocols